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Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology

Author

Listed:
  • Christopher A. Werley

    (Harvard University
    Q-State Biosciences)

  • Stefano Boccardo

    (Harvard University
    Nobel Biocare AG)

  • Alessandra Rigamonti

    (Karolinska Institute)

  • Emil M. Hansson

    (Karolinska Institute
    Harvard University)

  • Adam E. Cohen

    (Harvard University
    Howard Hughes Medical Institute)

Abstract

Cells typically respond to chemical or physical perturbations via complex signaling cascades which can simultaneously affect multiple physiological parameters, such as membrane voltage, calcium, pH, and redox potential. Protein-based fluorescent sensors can report many of these parameters, but spectral overlap prevents more than ~4 modalities from being recorded in parallel. Here we introduce the technique, MOSAIC, Multiplexed Optical Sensors in Arrayed Islands of Cells, where patterning of fluorescent sensor-encoding lentiviral vectors with a microarray printer enables parallel recording of multiple modalities. We demonstrate simultaneous recordings from 20 sensors in parallel in human embryonic kidney (HEK293) cells and in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), and we describe responses to metabolic and pharmacological perturbations. Together, these results show that MOSAIC can provide rich multi-modal data on complex physiological responses in multiple cell types.

Suggested Citation

  • Christopher A. Werley & Stefano Boccardo & Alessandra Rigamonti & Emil M. Hansson & Adam E. Cohen, 2020. "Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology," Nature Communications, Nature, vol. 11(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17607-5
    DOI: 10.1038/s41467-020-17607-5
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    Cited by:

    1. Yuzhen Zhang & Yukmi Cai & Bing Zhang & Yi-Heng P. Job Zhang, 2024. "Spatially structured exchange of metabolites enhances bacterial survival and resilience in biofilms," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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