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Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe

Author

Listed:
  • Xusan Yang

    (Peking University
    Cornell University)

  • Zhigang Yang

    (Shenzhen University)

  • Zhaoyang Wu

    (Peking University)

  • Ying He

    (Shenzhen University)

  • Chunyan Shan

    (Peking University
    Peking University)

  • Peiyuan Chai

    (Peking University)

  • Chenshuo Ma

    (Rutgers University)

  • Mi Tian

    (Shenzhen University)

  • Junlin Teng

    (Peking University)

  • Dayong Jin

    (Southern University of Science and Technology)

  • Wei Yan

    (Shenzhen University)

  • Pintu Das

    (Shenzhen University)

  • Junle Qu

    (Shenzhen University)

  • Peng Xi

    (Peking University
    Southern University of Science and Technology)

Abstract

Mitochondria play a critical role in generating energy to support the entire lifecycle of biological cells, yet it is still unclear how their morphological structures evolve to regulate their functionality. Conventional fluorescence microscopy can only provide ~300 nm resolution, which is insufficient to visualize mitochondrial cristae. Here, we developed an enhanced squaraine variant dye (MitoESq-635) to study the dynamic structures of mitochondrial cristae in live cells with a superresolution technique. The low saturation intensity and high photostability of MitoESq-635 make it ideal for long-term, high-resolution (stimulated emission depletion) STED nanoscopy. We performed time-lapse imaging of the mitochondrial inner membrane over 50 min (3.9 s per frame, with 71.5 s dark recovery) in living HeLa cells with a resolution of 35.2 nm. The forms of the cristae during mitochondrial fusion and fission can be clearly observed. Our study demonstrates the emerging capability of optical STED nanoscopy to investigate intracellular physiological processes with nanoscale resolution for an extended period of time.

Suggested Citation

  • Xusan Yang & Zhigang Yang & Zhaoyang Wu & Ying He & Chunyan Shan & Peiyuan Chai & Chenshuo Ma & Mi Tian & Junlin Teng & Dayong Jin & Wei Yan & Pintu Das & Junle Qu & Peng Xi, 2020. "Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17546-1
    DOI: 10.1038/s41467-020-17546-1
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    Cited by:

    1. Gangwei Jiang & Tian-Bing Ren & Elisa D’Este & Mengyi Xiong & Bin Xiong & Kai Johnsson & Xiao-Bing Zhang & Lu Wang & Lin Yuan, 2022. "A synergistic strategy to develop photostable and bright dyes with long Stokes shift for nanoscopy," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

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