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TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing

Author

Listed:
  • Theresa Schöpp

    (University of Edinburgh
    University of Edinburgh)

  • Ansgar Zoch

    (University of Edinburgh
    University of Edinburgh)

  • Rebecca V. Berrens

    (University of Cambridge, Li Ka Shing Centre, Robinson Way)

  • Tania Auchynnikava

    (University of Edinburgh)

  • Yuka Kabayama

    (University of Edinburgh
    University of Edinburgh)

  • Lina Vasiliauskaitė

    (University of Edinburgh)

  • Juri Rappsilber

    (University of Edinburgh
    Technische Universität Berlin)

  • Robin C. Allshire

    (University of Edinburgh)

  • Dónal O’Carroll

    (University of Edinburgh
    University of Edinburgh)

Abstract

The PIWI protein MIWI2 and its associated PIWI-interacting RNAs (piRNAs) instruct DNA methylation of young active transposable elements (TEs) in the male germline. piRNAs are proposed to recruit MIWI2 to the transcriptionally active TE loci by base pairing to nascent transcripts, however the downstream mechanisms and effector proteins utilized by MIWI2 in directing de novo TE methylation remain incompletely understood. Here, we show that MIWI2 associates with TEX15 in foetal gonocytes. TEX15 is predominantly a nuclear protein that is not required for piRNA biogenesis but is essential for piRNA-directed TE de novo methylation and silencing. In summary, TEX15 is an essential executor of mammalian piRNA-directed DNA methylation.

Suggested Citation

  • Theresa Schöpp & Ansgar Zoch & Rebecca V. Berrens & Tania Auchynnikava & Yuka Kabayama & Lina Vasiliauskaitė & Juri Rappsilber & Robin C. Allshire & Dónal O’Carroll, 2020. "TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing," Nature Communications, Nature, vol. 11(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17372-5
    DOI: 10.1038/s41467-020-17372-5
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