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Cryo-EM analysis of the post-fusion structure of the SARS-CoV spike glycoprotein

Author

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  • Xiaoyi Fan

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Duanfang Cao

    (Chinese Academy of Sciences)

  • Lingfei Kong

    (Chinese Academy of Sciences)

  • Xinzheng Zhang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Chinese Academy of Sciences)

Abstract

Global emergencies caused by the severe acute respiratory syndrome coronavirus (SARS-CoV), Middle-East respiratory syndrome coronavirus (MERS-CoV) and SARS-CoV-2 significantly endanger human health. The spike (S) glycoprotein is the key antigen and its conserved S2 subunit contributes to viral entry by mediating host-viral membrane fusion. However, structural information of the post-fusion S2 from these highly pathogenic human-infecting coronaviruses is still lacking. We used single-particle cryo-electron microscopy to show that the post-fusion SARS-CoV S2 forms a further rotated HR1-HR2 six-helix bundle and a tightly bound linker region upstream of the HR2 motif. The structures of pre- and post-fusion SARS-CoV S glycoprotein dramatically differ, resembling that of the Mouse hepatitis virus (MHV) and other class I viral fusion proteins. This structure suggests potential targets for the development of vaccines and therapies against a wide range of SARS-like coronaviruses.

Suggested Citation

  • Xiaoyi Fan & Duanfang Cao & Lingfei Kong & Xinzheng Zhang, 2020. "Cryo-EM analysis of the post-fusion structure of the SARS-CoV spike glycoprotein," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17371-6
    DOI: 10.1038/s41467-020-17371-6
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    Cited by:

    1. Ruipeng Lei & Enya Qing & Abby Odle & Meng Yuan & Chaminda D. Gunawardene & Timothy J. C. Tan & Natalie So & Wenhao O. Ouyang & Ian A. Wilson & Tom Gallagher & Stanley Perlman & Nicholas C. Wu & Lok-Y, 2024. "Functional and antigenic characterization of SARS-CoV-2 spike fusion peptide by deep mutational scanning," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Valeria Calvaresi & Antoni G. Wrobel & Joanna Toporowska & Dietmar Hammerschmid & Katie J. Doores & Richard T. Bradshaw & Ricardo B. Parsons & Donald J. Benton & Chloë Roustan & Eamonn Reading & Micha, 2023. "Structural dynamics in the evolution of SARS-CoV-2 spike glycoprotein," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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