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A general approach to engineer positive-going eFRET voltage indicators

Author

Listed:
  • Ahmed S. Abdelfattah

    (Howard Hughes Medical Institute)

  • Rosario Valenti

    (Howard Hughes Medical Institute)

  • Jihong Zheng

    (Howard Hughes Medical Institute)

  • Allan Wong

    (Howard Hughes Medical Institute)

  • Kaspar Podgorski

    (Howard Hughes Medical Institute)

  • Minoru Koyama

    (Howard Hughes Medical Institute)

  • Douglas S. Kim

    (Howard Hughes Medical Institute)

  • Eric R. Schreiter

    (Howard Hughes Medical Institute)

Abstract

Imaging membrane voltage from genetically defined cells offers the unique ability to report spatial and temporal dynamics of electrical signaling at cellular and circuit levels. Here, we present a general approach to engineer electrochromic fluorescence resonance energy transfer (eFRET) genetically encoded voltage indicators (GEVIs) with positive-going fluorescence response to membrane depolarization through rational manipulation of the native proton transport pathway in microbial rhodopsins. We transform the state-of-the-art eFRET GEVI Voltron into Positron, with kinetics and sensitivity equivalent to Voltron but flipped fluorescence signal polarity. We further apply this general approach to GEVIs containing different voltage sensitive rhodopsin domains and various fluorescent dye and fluorescent protein reporters.

Suggested Citation

  • Ahmed S. Abdelfattah & Rosario Valenti & Jihong Zheng & Allan Wong & Kaspar Podgorski & Minoru Koyama & Douglas S. Kim & Eric R. Schreiter, 2020. "A general approach to engineer positive-going eFRET voltage indicators," Nature Communications, Nature, vol. 11(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17322-1
    DOI: 10.1038/s41467-020-17322-1
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    Cited by:

    1. Arita Silapetere & Songhwan Hwang & Yusaku Hontani & Rodrigo G. Fernandez Lahore & Jens Balke & Francisco Velazquez Escobar & Martijn Tros & Patrick E. Konold & Rainer Matis & Roberta Croce & Peter J., 2022. "QuasAr Odyssey: the origin of fluorescence and its voltage sensitivity in microbial rhodopsins," Nature Communications, Nature, vol. 13(1), pages 1-20, December.

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