IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-17304-3.html
   My bibliography  Save this article

Global translation during early development depends on the essential transcription factor PRDM10

Author

Listed:
  • Brenda Y. Han

    (Technology and Research (A*STAR))

  • Michelle K. Y. Seah

    (Technology and Research (A*STAR))

  • Imogen R. Brooks

    (Technology and Research (A*STAR))

  • Delia H. P. Quek

    (Technology and Research (A*STAR))

  • Dominic R. Huxley

    (Technology and Research (A*STAR))

  • Chuan-Sheng Foo

    (Technology and Research (A*STAR))

  • Li Ting Lee

    (Technology and Research (A*STAR))

  • Heike Wollmann

    (Technology and Research (A*STAR))

  • Huili Guo

    (Technology and Research (A*STAR)
    National University of Singapore)

  • Daniel M. Messerschmidt

    (Technology and Research (A*STAR))

  • Ernesto Guccione

    (Technology and Research (A*STAR)
    Icahn School of Medicine at Mount Sinai)

Abstract

Members of the PR/SET domain-containing (PRDM) family of zinc finger transcriptional regulators play diverse developmental roles. PRDM10 is a yet uncharacterized family member, and its function in vivo is unknown. Here, we report an essential requirement for PRDM10 in pre-implantation embryos and embryonic stem cells (mESCs), where loss of PRDM10 results in severe cell growth inhibition. Detailed genomic and biochemical analyses reveal that PRDM10 functions as a sequence-specific transcription factor. We identify Eif3b, which encodes a core component of the eukaryotic translation initiation factor 3 (eIF3) complex, as a key downstream target, and demonstrate that growth inhibition in PRDM10-deficient mESCs is in part mediated through EIF3B-dependent effects on global translation. Our work elucidates the molecular function of PRDM10 in maintaining global translation, establishes its essential role in early embryonic development and mESC homeostasis, and offers insights into the functional repertoire of PRDMs as well as the transcriptional mechanisms regulating translation.

Suggested Citation

  • Brenda Y. Han & Michelle K. Y. Seah & Imogen R. Brooks & Delia H. P. Quek & Dominic R. Huxley & Chuan-Sheng Foo & Li Ting Lee & Heike Wollmann & Huili Guo & Daniel M. Messerschmidt & Ernesto Guccione, 2020. "Global translation during early development depends on the essential transcription factor PRDM10," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17304-3
    DOI: 10.1038/s41467-020-17304-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-17304-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-17304-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Jason A. Carter & Léonie Strömich & Matthew Peacey & Sarah R. Chapin & Lars Velten & Lars M. Steinmetz & Benedikt Brors & Sheena Pinto & Hannah V. Meyer, 2022. "Transcriptomic diversity in human medullary thymic epithelial cells," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17304-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.