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A molecular map of murine lymph node blood vascular endothelium at single cell resolution

Author

Listed:
  • Kevin Brulois

    (Stanford University School of Medicine)

  • Anusha Rajaraman

    (Stanford University School of Medicine
    Palo Alto Veterans Institute for Research
    Vrije Universiteit Medical Center)

  • Agata Szade

    (Stanford University School of Medicine
    Jagiellonian University)

  • Sofia Nordling

    (Stanford University School of Medicine)

  • Ania Bogoslowski

    (Cumming School of Medicine, University of Calgary
    Cumming School of Medicine, University of Calgary)

  • Denis Dermadi

    (Stanford University School of Medicine)

  • Milladur Rahman

    (Stanford University School of Medicine)

  • Helena Kiefel

    (Stanford University School of Medicine)

  • Edward O’Hara

    (Stanford University School of Medicine)

  • Jasper J. Koning

    (Vrije Universiteit Medical Center)

  • Hiroto Kawashima

    (Hoshi University)

  • Bin Zhou

    (Chinese Academy of Sciences)

  • Dietmar Vestweber

    (Max Planck Institute for Molecular Biomedicine)

  • Kristy Red-Horse

    (Stanford University)

  • Reina E. Mebius

    (Vrije Universiteit Medical Center)

  • Ralf H. Adams

    (University of Münster, Faculty of Medicine)

  • Paul Kubes

    (Cumming School of Medicine, University of Calgary
    Cumming School of Medicine, University of Calgary)

  • Junliang Pan

    (Stanford University School of Medicine
    Palo Alto Veterans Institute for Research)

  • Eugene C. Butcher

    (Stanford University School of Medicine
    Palo Alto Veterans Institute for Research
    Veterans Affairs Palo Alto Health Care System)

Abstract

Blood vascular endothelial cells (BECs) control the immune response by regulating blood flow and immune cell recruitment in lymphoid tissues. However, the diversity of BEC and their origins during immune angiogenesis remain unclear. Here we profile transcriptomes of BEC from peripheral lymph nodes and map phenotypes to the vasculature. We identify multiple subsets, including a medullary venous population whose gene signature predicts a selective role in myeloid cell (vs lymphocyte) recruitment to the medulla, confirmed by videomicroscopy. We define five capillary subsets, including a capillary resident precursor (CRP) that displays stem cell and migratory gene signatures, and contributes to homeostatic BEC turnover and to neogenesis of high endothelium after immunization. Cell alignments show retention of developmental programs along trajectories from CRP to mature venous and arterial populations. Our single cell atlas provides a molecular roadmap of the lymph node blood vasculature and defines subset specialization for leukocyte recruitment and vascular homeostasis.

Suggested Citation

  • Kevin Brulois & Anusha Rajaraman & Agata Szade & Sofia Nordling & Ania Bogoslowski & Denis Dermadi & Milladur Rahman & Helena Kiefel & Edward O’Hara & Jasper J. Koning & Hiroto Kawashima & Bin Zhou & , 2020. "A molecular map of murine lymph node blood vascular endothelium at single cell resolution," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17291-5
    DOI: 10.1038/s41467-020-17291-5
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    Cited by:

    1. Thanh Theresa Dinh & Menglan Xiang & Anusha Rajaraman & Yongzhi Wang & Nicole Salazar & Yu Zhu & Walter Roper & Siyeon Rhee & Kevin Brulois & Ed O’Hara & Helena Kiefel & Truc M. Dinh & Yuhan Bi & Dali, 2022. "An NKX-COUP-TFII morphogenetic code directs mucosal endothelial addressin expression," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Gopal Chovatiya & Kefei Nina Li & Jonathan Li & Sangeeta Ghuwalewala & Tudorita Tumbar, 2023. "Alk1 acts in non-endothelial VE-cadherin+ perineurial cells to maintain nerve branching during hair homeostasis," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Susanne Fleig & Tamar Kapanadze & Jeremiah Bernier-Latmani & Julia K. Lill & Tania Wyss & Jaba Gamrekelashvili & Dustin Kijas & Bin Liu & Anne M. Hüsing & Esther Bovay & Adan Chari Jirmo & Stephan Hal, 2022. "Loss of vascular endothelial notch signaling promotes spontaneous formation of tertiary lymphoid structures," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    4. Vincent Geldhof & Laura P. M. H. Rooij & Liliana Sokol & Jacob Amersfoort & Maxim Schepper & Katerina Rohlenova & Griet Hoste & Adriaan Vanderstichele & Anne-Marie Delsupehe & Edoardo Isnaldi & Naima , 2022. "Single cell atlas identifies lipid-processing and immunomodulatory endothelial cells in healthy and malignant breast," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    5. Huihui Liu & Hongchao Liu & Longhao Wang & Lei Song & Guixian Jiang & Qing Lu & Tao Yang & Hu Peng & Ruijie Cai & Xingle Zhao & Ting Zhao & Hao Wu, 2023. "Cochlear transcript diversity and its role in auditory functions implied by an otoferlin short isoform," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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