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In planta study of photosynthesis and photorespiration using NADPH and NADH/NAD+ fluorescent protein sensors

Author

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  • Shey-Li Lim

    (University of Hong Kong)

  • Chia Pao Voon

    (University of Hong Kong)

  • Xiaoqian Guan

    (University of Hong Kong)

  • Yi Yang

    (East China University of Science and Technology)

  • Per Gardeström

    (Umeå University)

  • Boon Leong Lim

    (University of Hong Kong
    The Chinese University of Hong Kong
    HKU Shenzhen Institute of Research and Innovation)

Abstract

The challenge of monitoring in planta dynamic changes of NADP(H) and NAD(H) redox states at the subcellular level is considered a major obstacle in plant bioenergetics studies. Here, we introduced two circularly permuted yellow fluorescent protein sensors, iNAP and SoNar, into Arabidopsis thaliana to monitor the dynamic changes in NADPH and the NADH/NAD+ ratio. In the light, photosynthesis and photorespiration are linked to the redox states of NAD(P)H and NAD(P) pools in several subcellular compartments connected by the malate-OAA shuttles. We show that the photosynthetic increases in stromal NADPH and NADH/NAD+ ratio, but not ATP, disappear when glycine decarboxylation is inhibited. These observations highlight the complex interplay between chloroplasts and mitochondria during photosynthesis and support the suggestions that, under normal conditions, photorespiration supplies a large amount of NADH to mitochondria, exceeding its NADH-dissipating capacity, and the surplus NADH is exported from the mitochondria to the cytosol through the malate-OAA shuttle.

Suggested Citation

  • Shey-Li Lim & Chia Pao Voon & Xiaoqian Guan & Yi Yang & Per Gardeström & Boon Leong Lim, 2020. "In planta study of photosynthesis and photorespiration using NADPH and NADH/NAD+ fluorescent protein sensors," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17056-0
    DOI: 10.1038/s41467-020-17056-0
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    Cited by:

    1. Shey-Li Lim & Sabrina Flütsch & Jinhong Liu & Luca Distefano & Diana Santelia & Boon Leong Lim, 2022. "Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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