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The phytopathogenic fungus Sclerotinia sclerotiorum detoxifies plant glucosinolate hydrolysis products via an isothiocyanate hydrolase

Author

Listed:
  • Jingyuan Chen

    (Max Planck Institute for Chemical Ecology)

  • Chhana Ullah

    (Max Planck Institute for Chemical Ecology)

  • Michael Reichelt

    (Max Planck Institute for Chemical Ecology)

  • Franziska Beran

    (Max Planck Institute for Chemical Ecology)

  • Zhi-Ling Yang

    (Max Planck Institute for Chemical Ecology)

  • Jonathan Gershenzon

    (Max Planck Institute for Chemical Ecology)

  • Almuth Hammerbacher

    (University of Pretoria)

  • Daniel G. Vassão

    (Max Planck Institute for Chemical Ecology)

Abstract

Brassicales plants produce glucosinolates and myrosinases that generate toxic isothiocyanates conferring broad resistance against pathogens and herbivorous insects. Nevertheless, some cosmopolitan fungal pathogens, such as the necrotrophic white mold Sclerotinia sclerotiorum, are able to infect many plant hosts including glucosinolate producers. Here, we show that S. sclerotiorum infection activates the glucosinolate-myrosinase system, and isothiocyanates contribute to resistance against this fungus. S. sclerotiorum metabolizes isothiocyanates via two independent pathways: conjugation to glutathione and, more effectively, hydrolysis to amines. The latter pathway features an isothiocyanate hydrolase that is homologous to a previously characterized bacterial enzyme, and converts isothiocyanate into products that are not toxic to the fungus. The isothiocyanate hydrolase promotes fungal growth in the presence of the toxins, and contributes to the virulence of S. sclerotiorum on glucosinolate-producing plants.

Suggested Citation

  • Jingyuan Chen & Chhana Ullah & Michael Reichelt & Franziska Beran & Zhi-Ling Yang & Jonathan Gershenzon & Almuth Hammerbacher & Daniel G. Vassão, 2020. "The phytopathogenic fungus Sclerotinia sclerotiorum detoxifies plant glucosinolate hydrolysis products via an isothiocyanate hydrolase," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16921-2
    DOI: 10.1038/s41467-020-16921-2
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    Cited by:

    1. Kerstin Unger & Syed Ali Komail Raza & Teresa Mayer & Michael Reichelt & Johannes Stuttmann & Annika Hielscher & Ute Wittstock & Jonathan Gershenzon & Matthew T. Agler, 2024. "Glucosinolate structural diversity shapes recruitment of a metabolic network of leaf-associated bacteria," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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