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Diauxie and co-utilization of carbon sources can coexist during bacterial growth in nutritionally complex environments

Author

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  • Elena Perrin

    (University of Florence)

  • Veronica Ghini

    (Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine (CIRMMP))

  • Michele Giovannini

    (University of Florence)

  • Francesca Di Patti

    (University of Florence
    CSDC, University of Florence)

  • Barbara Cardazzo

    (University of Padova)

  • Lisa Carraro

    (University of Padova)

  • Camilla Fagorzi

    (University of Florence)

  • Paola Turano

    (Center of Magnetic Resonance (CERM), University of Florence)

  • Renato Fani

    (University of Florence)

  • Marco Fondi

    (University of Florence
    CSDC, University of Florence)

Abstract

It is commonly thought that when multiple carbon sources are available, bacteria metabolize them either sequentially (diauxic growth) or simultaneously (co-utilization). However, this view is mainly based on analyses in relatively simple laboratory settings. Here we show that a heterotrophic marine bacterium, Pseudoalteromonas haloplanktis, can use both strategies simultaneously when multiple possible nutrients are provided in the same growth experiment. The order of nutrient uptake is partially determined by the biomass yield that can be achieved when the same compounds are provided as single carbon sources. Using transcriptomics and time-resolved intracellular 1H-13C NMR, we reveal specific pathways for utilization of various amino acids. Finally, theoretical modelling indicates that this metabolic phenotype, combining diauxie and co-utilization of substrates, is compatible with a tight regulation that allows the modulation of assimilatory pathways.

Suggested Citation

  • Elena Perrin & Veronica Ghini & Michele Giovannini & Francesca Di Patti & Barbara Cardazzo & Lisa Carraro & Camilla Fagorzi & Paola Turano & Renato Fani & Marco Fondi, 2020. "Diauxie and co-utilization of carbon sources can coexist during bacterial growth in nutritionally complex environments," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16872-8
    DOI: 10.1038/s41467-020-16872-8
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    Cited by:

    1. Zihan Wang & Akshit Goyal & Veronika Dubinkina & Ashish B. George & Tong Wang & Yulia Fridman & Sergei Maslov, 2021. "Complementary resource preferences spontaneously emerge in diauxic microbial communities," Nature Communications, Nature, vol. 12(1), pages 1-12, December.

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