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Spitzenkörper assembly mechanisms reveal conserved features of fungal and metazoan polarity scaffolds

Author

Listed:
  • Peng Zheng

    (National University of Singapore)

  • Tu Anh Nguyen

    (National University of Singapore)

  • Jie Yun Wong

    (National University of Singapore)

  • Michelle Lee

    (National University of Singapore)

  • The-Anh Nguyen

    (National University of Singapore)

  • Jing-Song Fan

    (National University of Singapore)

  • Daiwen Yang

    (National University of Singapore)

  • Gregory Jedd

    (National University of Singapore
    National University of Singapore)

Abstract

The Spitzenkörper (SPK) constitutes a collection of secretory vesicles and polarity-related proteins intimately associated with polarized growth of fungal hyphae. Many SPK-localized proteins are known, but their assembly and dynamics remain poorly understood. Here, we identify protein-protein interaction cascades leading to assembly of two SPK scaffolds and recruitment of diverse effectors in Neurospora crassa. Both scaffolds are transported to the SPK by the myosin V motor (MYO-5), with the coiled-coil protein SPZ-1 acting as cargo adaptor. Neither scaffold appears to be required for accumulation of SPK secretory vesicles. One scaffold consists of Leashin-2 (LAH-2), which is required for SPK localization of the signalling kinase COT-1 and the glycolysis enzyme GPI-1. The other scaffold comprises a complex of Janus-1 (JNS-1) and the polarisome protein SPA-2. Via its Spa homology domain (SHD), SPA-2 recruits a calponin domain-containing F-actin effector (CCP-1). The SHD NMR structure reveals a conserved surface groove required for effector binding. Similarities between SPA-2/JNS-1 and the metazoan GIT/PIX complex identify foundational features of the cell polarity apparatus that predate the fungal-metazoan divergence.

Suggested Citation

  • Peng Zheng & Tu Anh Nguyen & Jie Yun Wong & Michelle Lee & The-Anh Nguyen & Jing-Song Fan & Daiwen Yang & Gregory Jedd, 2020. "Spitzenkörper assembly mechanisms reveal conserved features of fungal and metazoan polarity scaffolds," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16712-9
    DOI: 10.1038/s41467-020-16712-9
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