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Sub-nanowatt resolution direct calorimetry for probing real-time metabolic activity of individual C. elegans worms

Author

Listed:
  • Sunghoon Hur

    (University of Michigan)

  • Rohith Mittapally

    (University of Michigan)

  • Swathi Yadlapalli

    (University of Michigan)

  • Pramod Reddy

    (University of Michigan
    University of Michigan)

  • Edgar Meyhofer

    (University of Michigan
    University of Michigan)

Abstract

Calorimetry has been widely used in metabolic studies, but direct measurements from individual small biological model organisms such as C. elegans or isolated single cells have been limited by poor sensitivity of existing techniques and difficulties in resolving very small heat outputs. Here, by careful thermal engineering, we developed a robust, highly sensitive and bio-compatible calorimetric platform that features a resolution of ~270 pW—more than a 500-fold improvement over the most sensitive calorimeter previously used for measuring the metabolic heat output of C. elegans. Using this calorimeter, we demonstrate time-resolved metabolic measurements of single C. elegans worms from larval to adult stages. Further, we show that the metabolic output is significantly lower in long-lived C. elegans daf-2 mutants. These demonstrations clearly highlight the broad potential of this tool for studying the role of metabolism in disease, development and aging of small model organisms and single cells.

Suggested Citation

  • Sunghoon Hur & Rohith Mittapally & Swathi Yadlapalli & Pramod Reddy & Edgar Meyhofer, 2020. "Sub-nanowatt resolution direct calorimetry for probing real-time metabolic activity of individual C. elegans worms," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16690-y
    DOI: 10.1038/s41467-020-16690-y
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