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TECPR1 promotes aggrephagy by direct recruitment of LC3C autophagosomes to lysosomes

Author

Listed:
  • Lisa Wetzel

    (Institute Pasteur
    Max Planck Institute of Biochemistry)

  • Stéphane Blanchard

    (Institute Pasteur)

  • Sowmya Rama

    (Institute Pasteur)

  • Viola Beier

    (Max Planck Institute of Biochemistry)

  • Anna Kaufmann

    (Max Planck Institute of Biochemistry)

  • Thomas Wollert

    (Institute Pasteur)

Abstract

The accumulation of protein aggregates is involved in the onset of many neurodegenerative diseases. Aggrephagy is a selective type of autophagy that counteracts neurodegeneration by degrading such aggregates. In this study, we found that LC3C cooperates with lysosomal TECPR1 to promote the degradation of disease-related protein aggregates in neural stem cells. The N-terminal WD-repeat domain of TECPR1 selectively binds LC3C which decorates matured autophagosomes. The interaction of LC3C and TECPR1 promotes the recruitment of autophagosomes to lysosomes for degradation. Augmented expression of TECPR1 in neural stem cells reduces the number of protein aggregates by promoting their autophagic clearance, whereas knockdown of LC3C inhibits aggrephagy. The PH domain of TECPR1 selectively interacts with PtdIns(4)P to target TECPR1 to PtdIns(4)P containing lysosomes. Exchanging the PH against a tandem-FYVE domain targets TECPR1 ectopically to endosomes. This leads to an accumulation of LC3C autophagosomes at endosomes and prevents their delivery to lysosomes.

Suggested Citation

  • Lisa Wetzel & Stéphane Blanchard & Sowmya Rama & Viola Beier & Anna Kaufmann & Thomas Wollert, 2020. "TECPR1 promotes aggrephagy by direct recruitment of LC3C autophagosomes to lysosomes," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16689-5
    DOI: 10.1038/s41467-020-16689-5
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