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ADP binding by the Culex quinquefasciatus mosquito D7 salivary protein enhances blood feeding on mammals

Author

Listed:
  • Ines Martin-Martin

    (National Institutes of Health)

  • Andrew Paige

    (National Institutes of Health)

  • Paola Carolina Valenzuela Leon

    (National Institutes of Health)

  • Apostolos G. Gittis

    (National Institutes of Health)

  • Olivia Kern

    (National Institutes of Health)

  • Brian Bonilla

    (National Institutes of Health)

  • Andrezza Campos Chagas

    (National Institutes of Health)

  • Sundar Ganesan

    (National Institutes of Health)

  • Leticia Barion Smith

    (National Institutes of Health)

  • David N. Garboczi

    (National Institutes of Health)

  • Eric Calvo

    (National Institutes of Health)

Abstract

During blood-feeding, mosquito saliva is injected into the skin to facilitate blood meal acquisition. D7 proteins are among the most abundant components of the mosquito saliva. Here we report the ligand binding specificity and physiological relevance of two D7 long proteins from Culex quinquefasciatus mosquito, the vector of filaria parasites or West Nile viruses. CxD7L2 binds biogenic amines and eicosanoids. CxD7L1 exhibits high affinity for ADP and ATP, a binding capacity not reported in any D7. We solve the crystal structure of CxD7L1 in complex with ADP to 1.97 Å resolution. The binding pocket lies between the two protein domains, whereas all known D7s bind ligands either within the N- or the C-terminal domains. We demonstrate that these proteins inhibit hemostasis in ex vivo and in vivo experiments. Our results suggest that the ADP-binding function acquired by CxD7L1 evolved to enhance blood-feeding in mammals, where ADP plays a key role in platelet aggregation.

Suggested Citation

  • Ines Martin-Martin & Andrew Paige & Paola Carolina Valenzuela Leon & Apostolos G. Gittis & Olivia Kern & Brian Bonilla & Andrezza Campos Chagas & Sundar Ganesan & Leticia Barion Smith & David N. Garbo, 2020. "ADP binding by the Culex quinquefasciatus mosquito D7 salivary protein enhances blood feeding on mammals," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16665-z
    DOI: 10.1038/s41467-020-16665-z
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    Cited by:

    1. Zarna Rajeshkumar Pala & Thiago Luiz Alves e Silva & Mahnaz Minai & Benjamin Crews & Eduardo Patino-Martinez & Carmelo Carmona-Rivera & Paola Carolina Valenzuela Leon & Ines Martin-Martin & Yevel Flor, 2024. "Mosquito salivary apyrase regulates blood meal hemostasis and facilitates malaria parasite transmission," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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