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Dicer-like 5 deficiency confers temperature-sensitive male sterility in maize

Author

Listed:
  • Chong Teng

    (Donald Danforth Plant Science Center)

  • Han Zhang

    (Stanford University)

  • Reza Hammond

    (University of Delaware)

  • Kun Huang

    (University of Delaware)

  • Blake C. Meyers

    (Donald Danforth Plant Science Center
    University of Missouri—Columbia)

  • Virginia Walbot

    (Stanford University)

Abstract

Small RNAs play important roles during plant development by regulating transcript levels of target mRNAs, maintaining genome integrity, and reinforcing DNA methylation. Dicer-like 5 (Dcl5) is proposed to be responsible for precise slicing in many monocots to generate diverse 24-nt phased, secondary small interfering RNAs (phasiRNAs), which are exceptionally abundant in meiotic anthers of diverse flowering plants. The importance and functions of these phasiRNAs remain unclear. Here, we characterized several mutants of dcl5, including alleles generated by the clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 system and a transposon-disrupted allele. We report that dcl5 mutants have few or no 24-nt phasiRNAs, develop short anthers with defective tapetal cells, and exhibit temperature-sensitive male fertility. We propose that DCL5 and 24-nt phasiRNAs are critical for fertility under growth regimes for optimal yield.

Suggested Citation

  • Chong Teng & Han Zhang & Reza Hammond & Kun Huang & Blake C. Meyers & Virginia Walbot, 2020. "Dicer-like 5 deficiency confers temperature-sensitive male sterility in maize," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16634-6
    DOI: 10.1038/s41467-020-16634-6
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