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AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex

Author

Listed:
  • Marscha Hirschi

    (The Scripps Research Institute)

  • Wang-Ting Lu

    (University of Toronto)

  • Andrew Santiago-Frangos

    (Montana State University)

  • Royce Wilkinson

    (Montana State University)

  • Sarah M. Golden

    (Montana State University)

  • Alan R. Davidson

    (University of Toronto)

  • Gabriel C. Lander

    (The Scripps Research Institute)

  • Blake Wiedenheft

    (Montana State University)

Abstract

Bacteria have evolved sophisticated adaptive immune systems, called CRISPR-Cas, that provide sequence-specific protection against phage infection. In turn, phages have evolved a broad spectrum of anti-CRISPRs that suppress these immune systems. Here we report structures of anti-CRISPR protein IF9 (AcrIF9) in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy). In addition to sterically blocking the hybridization of complementary dsDNA to the CRISPR RNA, our results show that AcrIF9 binding also promotes non-sequence-specific engagement with dsDNA, potentially sequestering the complex from target DNA. These findings highlight the versatility of anti-CRISPR mechanisms utilized by phages to suppress CRISPR-mediated immune systems.

Suggested Citation

  • Marscha Hirschi & Wang-Ting Lu & Andrew Santiago-Frangos & Royce Wilkinson & Sarah M. Golden & Alan R. Davidson & Gabriel C. Lander & Blake Wiedenheft, 2020. "AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex," Nature Communications, Nature, vol. 11(1), pages 1-6, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16512-1
    DOI: 10.1038/s41467-020-16512-1
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