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Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow

Author

Listed:
  • René Riedel

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association
    Max Planck Institute for Evolutionary Biology)

  • Richard Addo

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Marta Ferreira-Gomes

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Gitta Anne Heinz

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Frederik Heinrich

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Jannis Kummer

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Victor Greiff

    (Eidgenössische Technische Hochschule (ETH Zürich)
    University of Oslo)

  • Daniel Schulz

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Cora Klaeden

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Rebecca Cornelis

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Ulrike Menzel

    (Eidgenössische Technische Hochschule (ETH Zürich))

  • Stefan Kröger

    (Humboldt-Universität zu Berlin
    Robert Koch Institute)

  • Ulrik Stervbo

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association
    Charité-Universitätsmedizin Berlin)

  • Ralf Köhler

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Claudia Haftmann

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association
    Universitätsspital Zürich)

  • Silvia Kühnel

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Katrin Lehmann

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Patrick Maschmeyer

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Mairi McGrath

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Sandra Naundorf

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Stefanie Hahne

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Özen Sercan-Alp

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association
    Industriepark Hoechst)

  • Francesco Siracusa

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association
    University Medical Center Hamburg-Eppendorf)

  • Jonathan Stefanowski

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association
    Charité-Universitätsmedizin Berlin)

  • Melanie Weber

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Kerstin Westendorf

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Jakob Zimmermann

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association
    University of Bern)

  • Anja E. Hauser

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association
    Charité-Universitätsmedizin Berlin)

  • Sai T. Reddy

    (Eidgenössische Technische Hochschule (ETH Zürich))

  • Pawel Durek

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Hyun-Dong Chang

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

  • Mir-Farzin Mashreghi

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association
    BCRT/DRFZ Single-Cell Laboratory for Advanced Cellular Therapies - Brandenburg Center for Regenerative Therapies (BCRT))

  • Andreas Radbruch

    (Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association)

Abstract

At present, it is not clear how memory B lymphocytes are maintained over time, and whether only as circulating cells or also residing in particular tissues. Here we describe distinct populations of isotype-switched memory B lymphocytes (Bsm) of murine spleen and bone marrow, identified according to individual transcriptional signature and B cell receptor repertoire. A population of marginal zone-like cells is located exclusively in the spleen, while a population of quiescent Bsm is found only in the bone marrow. Three further resident populations, present in spleen and bone marrow, represent transitional and follicular B cells and B1 cells, respectively. A population representing 10-20% of spleen and bone marrow memory B cells is the only one qualifying as circulating. In the bone marrow, all cells individually dock onto VCAM1+ stromal cells and, reminiscent of resident memory T and plasma cells, are void of activation, proliferation and mobility.

Suggested Citation

  • René Riedel & Richard Addo & Marta Ferreira-Gomes & Gitta Anne Heinz & Frederik Heinrich & Jannis Kummer & Victor Greiff & Daniel Schulz & Cora Klaeden & Rebecca Cornelis & Ulrike Menzel & Stefan Krög, 2020. "Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16464-6
    DOI: 10.1038/s41467-020-16464-6
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    Cited by:

    1. Christopher M. Skopnik & René Riedel & Richard K. Addo & Gitta Anne Heinz & Frederik Heinrich & Kazuhito Honjo & Pawel Durek & Philipp Enghard & Mir-Farzin Mashreghi & Andreas Radbruch & Hiromi Kubaga, 2022. "Questioning whether IgM Fc receptor (FcµR) is expressed by innate immune cells," Nature Communications, Nature, vol. 13(1), pages 1-4, December.

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