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A SAM-I riboswitch with the ability to sense and respond to uncharged initiator tRNA

Author

Listed:
  • Dong-Jie Tang

    (Guangxi University)

  • Xinyu Du

    (Fudan University
    Fudan University Pudong Medical Center)

  • Qiang Shi

    (Zhongshan Hospital of Fudan University)

  • Jian-Ling Zhang

    (Guangxi University
    Zunyi Medical University)

  • Yuan-Ping He

    (Guangxi University)

  • Yan-Miao Chen

    (Guangxi University)

  • Zhenhua Ming

    (Guangxi University)

  • Dan Wang

    (Guangxi University)

  • Wan-Ying Zhong

    (Guangxi University)

  • Yu-Wei Liang

    (Guangxi University)

  • Jin-Yang Liu

    (Guangxi University)

  • Jian-Ming Huang

    (Fudan University Pudong Medical Center)

  • Yun-Shi Zhong

    (Zhongshan Hospital of Fudan University)

  • Shi-Qi An

    (University of Southampton)

  • Hongzhou Gu

    (Fudan University
    Fudan University Pudong Medical Center
    Zhongshan Hospital of Fudan University)

  • Ji-Liang Tang

    (Guangxi University)

Abstract

All known riboswitches use their aptamer to senese one metabolite signal and their expression platform to regulate gene expression. Here, we characterize a SAM-I riboswitch (SAM-IXcc) from the Xanthomonas campestris that regulates methionine synthesis via the met operon. In vitro and in vivo experiments show that SAM-IXcc controls the met operon primarily at the translational level in response to cellular S-adenosylmethionine (SAM) levels. Biochemical and genetic data demonstrate that SAM-IXcc expression platform not only can repress gene expression in response to SAM binding to SAM-IXcc aptamer but also can sense and bind uncharged initiator Met tRNA, resulting in the sequestering of the anti-Shine-Dalgarno (SD) sequence and freeing the SD for translation initiation. These findings identify a SAM-I riboswitch with a dual functioning expression platform that regulates methionine synthesis through a previously unrecognized mechanism and discover a natural tRNA-sensing RNA element. This SAM-I riboswitch appears to be highly conserved in Xanthomonas species.

Suggested Citation

  • Dong-Jie Tang & Xinyu Du & Qiang Shi & Jian-Ling Zhang & Yuan-Ping He & Yan-Miao Chen & Zhenhua Ming & Dan Wang & Wan-Ying Zhong & Yu-Wei Liang & Jin-Yang Liu & Jian-Ming Huang & Yun-Shi Zhong & Shi-Q, 2020. "A SAM-I riboswitch with the ability to sense and respond to uncharged initiator tRNA," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16417-z
    DOI: 10.1038/s41467-020-16417-z
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    Cited by:

    1. Yanyan Xue & Jun Li & Dian Chen & Xizhu Zhao & Liang Hong & Yu Liu, 2023. "Observation of structural switch in nascent SAM-VI riboswitch during transcription at single-nucleotide and single-molecule resolution," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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