IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-16214-8.html
   My bibliography  Save this article

Unique properties of a subset of human pluripotent stem cells with high capacity for self-renewal

Author

Listed:
  • Kevin X. Lau

    (University of Melbourne)

  • Elizabeth A. Mason

    (University of Melbourne
    University of Melbourne)

  • Joshua Kie

    (University of Melbourne)

  • David P. Souza

    (University of Melbourne)

  • Joachim Kloehn

    (University of Melbourne)

  • Dedreia Tull

    (University of Melbourne)

  • Malcolm J. McConville

    (University of Melbourne
    University of Melbourne)

  • Andrew Keniry

    (The Walter and Eliza Hall Institute
    University of Melbourne)

  • Tamara Beck

    (The Walter and Eliza Hall Institute)

  • Marnie E. Blewitt

    (The Walter and Eliza Hall Institute
    University of Melbourne)

  • Matthew E. Ritchie

    (The Walter and Eliza Hall Institute)

  • Shalin H. Naik

    (The Walter and Eliza Hall Institute)

  • Daniela Zalcenstein

    (The Walter and Eliza Hall Institute)

  • Othmar Korn

    (The University of Queensland)

  • Shian Su

    (The Walter and Eliza Hall Institute)

  • Irene Gallego Romero

    (University of Melbourne)

  • Catrina Spruce

    (The Jackson Laboratory)

  • Christopher L. Baker

    (The Jackson Laboratory)

  • Tracy C. McGarr

    (The Jackson Laboratory)

  • Christine A. Wells

    (University of Melbourne
    University of Melbourne
    The Walter and Eliza Hall Institute)

  • Martin F. Pera

    (University of Melbourne
    The Walter and Eliza Hall Institute
    The Jackson Laboratory
    The Florey Institute of Neuroscience and Mental Health)

Abstract

Archetypal human pluripotent stem cells (hPSC) are widely considered to be equivalent in developmental status to mouse epiblast stem cells, which correspond to pluripotent cells at a late post-implantation stage of embryogenesis. Heterogeneity within hPSC cultures complicates this interspecies comparison. Here we show that a subpopulation of archetypal hPSC enriched for high self-renewal capacity (ESR) has distinct properties relative to the bulk of the population, including a cell cycle with a very low G1 fraction and a metabolomic profile that reflects a combination of oxidative phosphorylation and glycolysis. ESR cells are pluripotent and capable of differentiation into primordial germ cell-like cells. Global DNA methylation levels in the ESR subpopulation are lower than those in mouse epiblast stem cells. Chromatin accessibility analysis revealed a unique set of open chromatin sites in ESR cells. RNA-seq at the subpopulation and single cell levels shows that, unlike mouse epiblast stem cells, the ESR subset of hPSC displays no lineage priming, and that it can be clearly distinguished from gastrulating and extraembryonic cell populations in the primate embryo. ESR hPSC correspond to an earlier stage of post-implantation development than mouse epiblast stem cells.

Suggested Citation

  • Kevin X. Lau & Elizabeth A. Mason & Joshua Kie & David P. Souza & Joachim Kloehn & Dedreia Tull & Malcolm J. McConville & Andrew Keniry & Tamara Beck & Marnie E. Blewitt & Matthew E. Ritchie & Shalin , 2020. "Unique properties of a subset of human pluripotent stem cells with high capacity for self-renewal," Nature Communications, Nature, vol. 11(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16214-8
    DOI: 10.1038/s41467-020-16214-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-16214-8
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-16214-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Timothy D. Arthur & Jennifer P. Nguyen & Agnieszka D’Antonio-Chronowska & Hiroko Matsui & Nayara S. Silva & Isaac N. Joshua & André D. Luchessi & William W. Young Greenwald & Matteo D’Antonio & Martin, 2024. "Complex regulatory networks influence pluripotent cell state transitions in human iPSCs," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16214-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.