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Multiplex precise base editing in cynomolgus monkeys

Author

Listed:
  • Wenhui Zhang

    (South China Agricultural University
    Guangdong Laboratory of Lingnan Modern Agriculture)

  • Tomomi Aida

    (Massachusetts Institute of Technology)

  • Ricardo C. H. Rosario

    (Broad Institute of MIT and Harvard)

  • Jonathan J. Wilde

    (Massachusetts Institute of Technology)

  • Chenhui Ding

    (Reproductive Medicine Center, the First Affiliated Hospital of Sun Yat-sen University)

  • Xiaohui Zhang

    (East China Normal University)

  • Zulqurain Baloch

    (South China Agricultural University)

  • Yan Huang

    (South China Agricultural University)

  • Yu Tang

    (South China Agricultural University)

  • Duanduan Li

    (South China Agricultural University)

  • Hongyu Lu

    (South China Agricultural University)

  • Yang Zhou

    (Massachusetts Institute of Technology)

  • Minqing Jiang

    (Massachusetts Institute of Technology)

  • Dongdong Xu

    (South China Agricultural University)

  • Zhihao Fang

    (South China Agricultural University)

  • Zhanhong Zheng

    (South China Agricultural University
    Guangdong Laboratory of Lingnan Modern Agriculture)

  • Qunshan Huang

    (South China Agricultural University)

  • Guoping Feng

    (Massachusetts Institute of Technology
    Broad Institute of MIT and Harvard)

  • Shihua Yang

    (South China Agricultural University
    Guangdong Laboratory of Lingnan Modern Agriculture)

Abstract

Common polygenic diseases result from compounded risk contributed by multiple genetic variants, meaning that simultaneous correction or introduction of single nucleotide variants is required for disease modeling and gene therapy. Here, we show precise, efficient, and simultaneous multiplex base editing of up to three target sites across 11 genes/loci in cynomolgus monkey embryos using CRISPR-based cytidine- and adenine-base editors. Unbiased whole genome sequencing demonstrates high specificity of base editing in monkey embryos. Our data demonstrate feasibility of multiplex base editing for polygenic disease modeling in primate zygotes.

Suggested Citation

  • Wenhui Zhang & Tomomi Aida & Ricardo C. H. Rosario & Jonathan J. Wilde & Chenhui Ding & Xiaohui Zhang & Zulqurain Baloch & Yan Huang & Yu Tang & Duanduan Li & Hongyu Lu & Yang Zhou & Minqing Jiang & D, 2020. "Multiplex precise base editing in cynomolgus monkeys," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16173-0
    DOI: 10.1038/s41467-020-16173-0
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    Cited by:

    1. Monjurul Hasan, A S M & Trianni, Andrea & Shukla, Nagesh & Katic, Mile, 2022. "A novel characterization based framework to incorporate industrial energy management services," Applied Energy, Elsevier, vol. 313(C).
    2. Qichen Yuan & Xue Gao, 2022. "Multiplex base- and prime-editing with drive-and-process CRISPR arrays," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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