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Par complex cluster formation mediated by phase separation

Author

Listed:
  • Ziheng Liu

    (Fudan University)

  • Ying Yang

    (National University of Singapore)

  • Aihong Gu

    (Fudan University)

  • Jiawen Xu

    (Fudan University)

  • Ying Mao

    (Fudan University)

  • Haojie Lu

    (Fudan University)

  • Weiguo Hu

    (Fudan University
    Fudan University)

  • Qun-Ying Lei

    (Fudan University)

  • Zhouhua Li

    (Capital Normal University)

  • Mingjie Zhang

    (Hong Kong University of Science and Technology)

  • Yu Cai

    (National University of Singapore)

  • Wenyu Wen

    (Fudan University)

Abstract

The evolutionarily conserved Par3/Par6/aPKC complex regulates the polarity establishment of diverse cell types and distinct polarity-driven functions. However, how the Par complex is concentrated beneath the membrane to initiate cell polarization remains unclear. Here we show that the Par complex exhibits cell cycle-dependent condensation in Drosophila neuroblasts, driven by liquid–liquid phase separation. The open conformation of Par3 undergoes autonomous phase separation likely due to its NTD-mediated oligomerization. Par6, via C-terminal tail binding to Par3 PDZ3, can be enriched to Par3 condensates and in return dramatically promote Par3 phase separation. aPKC can also be concentrated to the Par3N/Par6 condensates as a client. Interestingly, activated aPKC can disperse the Par3/Par6 condensates via phosphorylation of Par3. Perturbations of Par3/Par6 phase separation impair the establishment of apical–basal polarity during neuroblast asymmetric divisions and lead to defective lineage development. We propose that phase separation may be a common mechanism for localized cortical condensation of cell polarity complexes.

Suggested Citation

  • Ziheng Liu & Ying Yang & Aihong Gu & Jiawen Xu & Ying Mao & Haojie Lu & Weiguo Hu & Qun-Ying Lei & Zhouhua Li & Mingjie Zhang & Yu Cai & Wenyu Wen, 2020. "Par complex cluster formation mediated by phase separation," Nature Communications, Nature, vol. 11(1), pages 1-18, December.
  • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16135-6
    DOI: 10.1038/s41467-020-16135-6
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    Cited by:

    1. Rui Chen & Xinyao Shi & Xiangrui Yao & Tong Gao & Guangyu Huang & Duo Ning & Zemin Cao & Youxin Xu & Weizheng Liang & Simon Zhongyuan Tian & Qionghua Zhu & Liang Fang & Meizhen Zheng & Yuhui Hu & Huan, 2024. "Specific multivalent molecules boost CRISPR-mediated transcriptional activation," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Xuanyan Jia & Leishu Lin & Siqi Guo & Lulu Zhou & Gaowei Jin & Jiayuan Dong & Jinman Xiao & Xingqiao Xie & Yiming Li & Sicong He & Zhiyi Wei & Cong Yu, 2024. "CLASP-mediated competitive binding in protein condensates directs microtubule growth," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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