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Budding yeast complete DNA synthesis after chromosome segregation begins

Author

Listed:
  • Tsvetomira Ivanova

    (The Barcelona Institute of Science and Technology
    Universitat Pompeu Fabra (UPF))

  • Michael Maier

    (The Barcelona Institute of Science and Technology
    Universitat Pompeu Fabra (UPF))

  • Alsu Missarova

    (Universitat Pompeu Fabra (UPF))

  • Céline Ziegler-Birling

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire)

  • Monica Dam

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire)

  • Mercè Gomar-Alba

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire)

  • Lucas B. Carey

    (Universitat Pompeu Fabra (UPF)
    Peking University)

  • Manuel Mendoza

    (The Barcelona Institute of Science and Technology
    Universitat Pompeu Fabra (UPF)
    Institut de Génétique et de Biologie Moléculaire et Cellulaire
    Centre National de la Recherche Scientifique)

Abstract

To faithfully transmit genetic information, cells must replicate their entire genome before division. This is thought to be ensured by the temporal separation of replication and chromosome segregation. Here we show that in 20–40% of unperturbed yeast cells, DNA synthesis continues during anaphase, late in mitosis. High cyclin-Cdk activity inhibits DNA synthesis in metaphase, and the decrease in cyclin-Cdk activity during mitotic exit allows DNA synthesis to finish at subtelomeric and some difficult-to-replicate regions. DNA synthesis during late mitosis correlates with elevated mutation rates at subtelomeric regions, including copy number variation. Thus, yeast cells temporally overlap DNA synthesis and chromosome segregation during normal growth, possibly allowing cells to maximize population-level growth rate while simultaneously exploring greater genetic space.

Suggested Citation

  • Tsvetomira Ivanova & Michael Maier & Alsu Missarova & Céline Ziegler-Birling & Monica Dam & Mercè Gomar-Alba & Lucas B. Carey & Manuel Mendoza, 2020. "Budding yeast complete DNA synthesis after chromosome segregation begins," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16100-3
    DOI: 10.1038/s41467-020-16100-3
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