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The Set1 N-terminal domain and Swd2 interact with RNA polymerase II CTD to recruit COMPASS

Author

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  • Hyun Jin Bae

    (Harvard Medical School)

  • Marion Dubarry

    (UMR7258 CNRS, Aix Marseille University (AMU), Institut Paoli-Calmettes. Equipe labellisée Ligue contre le cancer)

  • Jongcheol Jeon

    (Harvard Medical School
    Korea Advanced Institute of Science and Technology)

  • Luis M. Soares

    (Harvard Medical School
    Foghorn Therapeutics)

  • Catherine Dargemont

    (UMR 9002 CNRS)

  • Jaehoon Kim

    (Korea Advanced Institute of Science and Technology)

  • Vincent Geli

    (UMR7258 CNRS, Aix Marseille University (AMU), Institut Paoli-Calmettes. Equipe labellisée Ligue contre le cancer)

  • Stephen Buratowski

    (Harvard Medical School)

Abstract

Methylation of histone H3 lysine 4 (H3K4) by Set1/COMPASS occurs co-transcriptionally, and is important for gene regulation. Set1/COMPASS associates with the RNA polymerase II C-terminal domain (CTD) to establish proper levels and distribution of H3K4 methylations. However, details of CTD association remain unclear. Here we report that the Set1 N-terminal region and the COMPASS subunit Swd2, which interact with each other, are both needed for efficient CTD binding in Saccharomyces cerevisiae. Moreover, a single point mutation in Swd2 that affects its interaction with Set1 also impairs COMPASS recruitment to chromatin and H3K4 methylation. A CTD interaction domain (CID) from the protein Nrd1 can partially substitute for the Set1 N-terminal region to restore CTD interactions and histone methylation. However, even when Set1/COMPASS is recruited via the Nrd1 CID, histone H2B ubiquitylation is still required for efficient H3K4 methylation, indicating that H2Bub acts after the initial recruitment of COMPASS to chromatin.

Suggested Citation

  • Hyun Jin Bae & Marion Dubarry & Jongcheol Jeon & Luis M. Soares & Catherine Dargemont & Jaehoon Kim & Vincent Geli & Stephen Buratowski, 2020. "The Set1 N-terminal domain and Swd2 interact with RNA polymerase II CTD to recruit COMPASS," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16082-2
    DOI: 10.1038/s41467-020-16082-2
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    Cited by:

    1. Nagham Ghaddar & Yves Corda & Pierre Luciano & Martina Galli & Ylli Doksani & Vincent Géli, 2023. "The COMPASS subunit Spp1 protects nascent DNA at the Tus/Ter replication fork barrier by limiting DNA availability to nucleases," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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