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LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development

Author

Listed:
  • Nicholas K. Vinckier

    (University of California, San Diego)

  • Nisha A. Patel

    (University of California, San Diego)

  • Ryan J. Geusz

    (University of California, San Diego)

  • Allen Wang

    (University of California, San Diego)

  • Jinzhao Wang

    (University of California, San Diego)

  • Ileana Matta

    (University of California, San Diego)

  • Austin R. Harrington

    (University of California, San Diego)

  • Matthew Wortham

    (University of California, San Diego)

  • Nichole Wetton

    (University of California, San Diego)

  • Jianxun Wang

    (University of California, San Diego)

  • Ulupi S. Jhala

    (University of California, San Diego)

  • Michael G. Rosenfeld

    (University of California, San Diego)

  • Christopher W. Benner

    (University of California, San Diego)

  • Hung-Ping Shih

    (Diabetes & Metabolism Research Institute, City of Hope)

  • Maike Sander

    (University of California, San Diego)

Abstract

Developmental progression depends on temporally defined changes in gene expression mediated by transient exposure of lineage intermediates to signals in the progenitor niche. To determine whether cell-intrinsic epigenetic mechanisms contribute to signal-induced transcriptional responses, here we manipulate the signalling environment and activity of the histone demethylase LSD1 during differentiation of hESC-gut tube intermediates into pancreatic endocrine cells. We identify a transient requirement for LSD1 in endocrine cell differentiation spanning a short time-window early in pancreas development, a phenotype we reproduced in mice. Examination of enhancer and transcriptome landscapes revealed that LSD1 silences transiently active retinoic acid (RA)-induced enhancers and their target genes. Furthermore, prolonged RA exposure phenocopies LSD1 inhibition, suggesting that LSD1 regulates endocrine cell differentiation by limiting the duration of RA signalling. Our findings identify LSD1-mediated enhancer silencing as a cell-intrinsic epigenetic feedback mechanism by which the duration of the transcriptional response to a developmental signal is limited.

Suggested Citation

  • Nicholas K. Vinckier & Nisha A. Patel & Ryan J. Geusz & Allen Wang & Jinzhao Wang & Ileana Matta & Austin R. Harrington & Matthew Wortham & Nichole Wetton & Jianxun Wang & Ulupi S. Jhala & Michael G. , 2020. "LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16017-x
    DOI: 10.1038/s41467-020-16017-x
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