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Intracellular Staphylococcus aureus persisters upon antibiotic exposure

Author

Listed:
  • Frédéric Peyrusson

    (Université catholique de Louvain (UCLouvain))

  • Hugo Varet

    (Institut Pasteur, USR 3756 CNRS)

  • Tiep Khac Nguyen

    (Université catholique de Louvain (UCLouvain))

  • Rachel Legendre

    (Institut Pasteur, USR 3756 CNRS)

  • Odile Sismeiro

    (Centre de Ressources et Recherches Technologiques (C2RT))

  • Jean-Yves Coppée

    (Centre de Ressources et Recherches Technologiques (C2RT))

  • Christiane Wolz

    (Institut für Medizinische Mikrobiologie und Hygiene)

  • Tanel Tenson

    (University of Tartu)

  • Françoise Van Bambeke

    (Université catholique de Louvain (UCLouvain))

Abstract

Bacterial persister cells are phenotypic variants that exhibit a transient non-growing state and antibiotic tolerance. Here, we provide in vitro evidence of Staphylococcus aureus persisters within infected host cells. We show that the bacteria surviving antibiotic treatment within host cells are persisters, displaying biphasic killing and reaching a uniformly non-responsive, non-dividing state when followed at the single-cell level. This phenotype is stable but reversible upon antibiotic removal. Intracellular S. aureus persisters remain metabolically active but display an altered transcriptomic profile consistent with activation of stress responses, including the stringent response as well as cell wall stress, SOS and heat shock responses. These changes are associated with multidrug tolerance after exposure to a single antibiotic. We hypothesize that intracellular S. aureus persisters may constitute a reservoir for relapsing infection and could contribute to therapeutic failures.

Suggested Citation

  • Frédéric Peyrusson & Hugo Varet & Tiep Khac Nguyen & Rachel Legendre & Odile Sismeiro & Jean-Yves Coppée & Christiane Wolz & Tanel Tenson & Françoise Van Bambeke, 2020. "Intracellular Staphylococcus aureus persisters upon antibiotic exposure," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15966-7
    DOI: 10.1038/s41467-020-15966-7
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    Cited by:

    1. Elizabeth V. K. Ledger & Stéphane Mesnage & Andrew M. Edwards, 2022. "Human serum triggers antibiotic tolerance in Staphylococcus aureus," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    2. Amir Arastehfar & Farnaz Daneshnia & Nathaly Cabrera & Suyapa Penalva-Lopez & Jansy Sarathy & Matthew Zimmerman & Erika Shor & David S. Perlin, 2023. "Macrophage internalization creates a multidrug-tolerant fungal persister reservoir and facilitates the emergence of drug resistance," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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