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Olfactory marker protein directly buffers cAMP to avoid depolarization-induced silencing of olfactory receptor neurons

Author

Listed:
  • Noriyuki Nakashima

    (Kurume University School of Medicine
    Kyoto University)

  • Kie Nakashima

    (Kyoto University
    Kyoto University)

  • Akiko Taura

    (Kyoto University Hospital
    Aino University)

  • Akiko Takaku-Nakashima

    (Kurume University School of Medicine
    The University of Tokyo Hospital)

  • Harunori Ohmori

    (Kyoto University
    Kanazawa Medical University)

  • Makoto Takano

    (Kurume University School of Medicine)

Abstract

Olfactory receptor neurons (ORNs) use odour-induced intracellular cAMP surge to gate cyclic nucleotide-gated nonselective cation (CNG) channels in cilia. Prolonged exposure to cAMP causes calmodulin-dependent feedback-adaptation of CNG channels and attenuates neural responses. On the other hand, the odour-source searching behaviour requires ORNs to be sensitive to odours when approaching targets. How ORNs accommodate these conflicting aspects of cAMP responses remains unknown. Here, we discover that olfactory marker protein (OMP) is a major cAMP buffer that maintains the sensitivity of ORNs. Upon the application of sensory stimuli, OMP directly captured and swiftly reduced freely available cAMP, which transiently uncoupled downstream CNG channel activity and prevented persistent depolarization. Under repetitive stimulation, OMP-/- ORNs were immediately silenced after burst firing due to sustained depolarization and inactivated firing machinery. Consequently, OMP-/- mice showed serious impairment in odour-source searching tasks. Therefore, cAMP buffering by OMP maintains the resilient firing of ORNs.

Suggested Citation

  • Noriyuki Nakashima & Kie Nakashima & Akiko Taura & Akiko Takaku-Nakashima & Harunori Ohmori & Makoto Takano, 2020. "Olfactory marker protein directly buffers cAMP to avoid depolarization-induced silencing of olfactory receptor neurons," Nature Communications, Nature, vol. 11(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15917-2
    DOI: 10.1038/s41467-020-15917-2
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    Cited by:

    1. Devendra Kumar Maurya & Anna Berghard & Staffan Bohm, 2022. "A multivesicular body-like organelle mediates stimulus-regulated trafficking of olfactory ciliary transduction proteins," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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