IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-15618-w.html
   My bibliography  Save this article

Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates

Author

Listed:
  • Sebastian Karpf

    (University of California, Los Angeles (UCLA)
    University of Luebeck)

  • Carson T. Riche

    (University of California, Los Angeles (UCLA))

  • Dino Carlo

    (University of California, Los Angeles (UCLA))

  • Anubhuti Goel

    (University of California, Los Angeles (UCLA))

  • William A. Zeiger

    (University of California, Los Angeles (UCLA))

  • Anand Suresh

    (University of California, Los Angeles (UCLA))

  • Carlos Portera-Cailliau

    (University of California, Los Angeles (UCLA))

  • Bahram Jalali

    (University of California, Los Angeles (UCLA)
    University of California, Los Angeles (UCLA))

Abstract

Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging speeds, however, mechanical scanners still limit the acquisition rates to typically 10–100 frames per second. Here we present a high-speed non-linear microscope achieving kilohertz frame rates by employing pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion. In combination with a high bandwidth, single-photon sensitive detector, this enables recording of fluorescent lifetimes at speeds of 88 million pixels per second. We show high resolution, multi-modal - two-photon fluorescence and fluorescence lifetime (FLIM) – microscopy and imaging flow cytometry with a digitally reconfigurable laser, imaging system and data acquisition system. These high speeds should enable high-speed and high-throughput image-assisted cell sorting.

Suggested Citation

  • Sebastian Karpf & Carson T. Riche & Dino Carlo & Anubhuti Goel & William A. Zeiger & Anand Suresh & Carlos Portera-Cailliau & Bahram Jalali, 2020. "Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15618-w
    DOI: 10.1038/s41467-020-15618-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-15618-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-15618-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15618-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.