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Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase

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  • Christin Radon

    (University of Potsdam)

  • Gerd Mittelstädt

    (University of Potsdam
    Victoria University of Wellington)

  • Benjamin R. Duffus

    (University of Potsdam)

  • Jörg Bürger

    (Max-Planck Institute of Molecular Genetics
    Charité, Institut für Medizinische Physik und Biophysik)

  • Tobias Hartmann

    (University of Potsdam)

  • Thorsten Mielke

    (Max-Planck Institute of Molecular Genetics)

  • Christian Teutloff

    (Freie Universität Berlin)

  • Silke Leimkühler

    (University of Potsdam)

  • Petra Wendler

    (University of Potsdam)

Abstract

Metal-containing formate dehydrogenases (FDH) catalyse the reversible oxidation of formate to carbon dioxide at their molybdenum or tungsten active site. They display a diverse subunit and cofactor composition, but structural information on these enzymes is limited. Here we report the cryo-electron microscopic structures of the soluble Rhodobacter capsulatus FDH (RcFDH) as isolated and in the presence of reduced nicotinamide adenine dinucleotide (NADH). RcFDH assembles into a 360 kDa dimer of heterotetramers revealing a putative interconnection of electron pathway chains. In the presence of NADH, the RcFDH structure shows charging of cofactors, indicative of an increased electron load.

Suggested Citation

  • Christin Radon & Gerd Mittelstädt & Benjamin R. Duffus & Jörg Bürger & Tobias Hartmann & Thorsten Mielke & Christian Teutloff & Silke Leimkühler & Petra Wendler, 2020. "Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15614-0
    DOI: 10.1038/s41467-020-15614-0
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    Cited by:

    1. Ji, Xiuling & Guo, Hao & Xue, Yaju & Huang, Yuhong & Zhang, Suojiang, 2023. "Microenvironment: An efficient avenue for converting CO2 to high-value compounds," Renewable and Sustainable Energy Reviews, Elsevier, vol. 188(C).

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