IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-15577-2.html
   My bibliography  Save this article

Identification of a phosphorylation site on Ulk1 required for genotoxic stress-induced alternative autophagy

Author

Listed:
  • Satoru Torii

    (Tokyo Medical and Dental University (TMDU))

  • Hirofumi Yamaguchi

    (Tokyo Medical and Dental University (TMDU))

  • Akira Nakanishi

    (Tokyo Medical and Dental University (TMDU))

  • Satoko Arakawa

    (Tokyo Medical and Dental University (TMDU))

  • Shinya Honda

    (Tokyo Medical and Dental University (TMDU))

  • Kenta Moriwaki

    (Osaka University)

  • Hiroyasu Nakano

    (Toho University School of Medicine)

  • Shigeomi Shimizu

    (Tokyo Medical and Dental University (TMDU))

Abstract

Alternative autophagy is an autophagy-related protein 5 (Atg5)-independent type of macroautophagy. Unc51-like kinase 1 (Ulk1) is an essential initiator not only for Atg5-dependent canonical autophagy but also for alternative autophagy. However, the mechanism as to how Ulk1 differentially regulates both types of autophagy has remained unclear. In this study, we identify a phosphorylation site of Ulk1 at Ser746, which is phosphorylated during genotoxic stress-induced alternative autophagy. Phospho-Ulk1746 localizes exclusively on the Golgi and is required for alternative autophagy, but not canonical autophagy. We also identify receptor-interacting protein kinase 3 (RIPK3) as the kinase responsible for genotoxic stress-induced Ulk1746 phosphorylation, because RIPK3 interacts with and phosphorylates Ulk1 at Ser746, and loss of RIPK3 abolishes Ulk1746 phosphorylation. These findings indicate that RIPK3-dependent Ulk1746 phosphorylation on the Golgi plays a pivotal role in genotoxic stress-induced alternative autophagy.

Suggested Citation

  • Satoru Torii & Hirofumi Yamaguchi & Akira Nakanishi & Satoko Arakawa & Shinya Honda & Kenta Moriwaki & Hiroyasu Nakano & Shigeomi Shimizu, 2020. "Identification of a phosphorylation site on Ulk1 required for genotoxic stress-induced alternative autophagy," Nature Communications, Nature, vol. 11(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15577-2
    DOI: 10.1038/s41467-020-15577-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-15577-2
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-15577-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15577-2. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.