IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-15408-4.html
   My bibliography  Save this article

Angiotensin II induces coordinated calcium bursts in aldosterone-producing adrenal rosettes

Author

Listed:
  • Nick A. Guagliardo

    (Departments of Pharmacology)

  • Peter M. Klein

    (Departments of Pharmacology
    University of Virginia)

  • Christina A. Gancayco

    (University of Virginia)

  • Adam Lu

    (Departments of Pharmacology
    University of Virginia)

  • Sining Leng

    (Boston Children’s Hospital)

  • Rany R. Makarem

    (Departments of Pharmacology)

  • Chelsea Cho

    (Departments of Pharmacology)

  • Craig G. Rusin

    (Baylor College of Medicine, and Harvard Stem Cell Institute)

  • David T. Breault

    (Boston Children’s Hospital)

  • Paula Q. Barrett

    (Departments of Pharmacology)

  • Mark P. Beenhakker

    (Departments of Pharmacology
    University of Virginia)

Abstract

Aldosterone-producing zona glomerulosa (zG) cells of the adrenal gland arrange in distinct multi-cellular rosettes that provide a structural framework for adrenal cortex morphogenesis and plasticity. Whether this cyto-architecture also plays functional roles in signaling remains unexplored. To determine if structure informs function, we generated mice with zG-specific expression of GCaMP3 and imaged zG cells within their native rosette structure. Here we demonstrate that within the rosette, angiotensin II evokes periodic Cav3-dependent calcium events that form bursts that are stereotypic in form. Our data reveal a critical role for angiotensin II in regulating burst occurrence, and a multifunctional role for the rosette structure in activity-prolongation and coordination. Combined our data define the calcium burst as the fundamental unit of zG layer activity evoked by angiotensin II and highlight a novel role for the rosette as a facilitator of cell communication.

Suggested Citation

  • Nick A. Guagliardo & Peter M. Klein & Christina A. Gancayco & Adam Lu & Sining Leng & Rany R. Makarem & Chelsea Cho & Craig G. Rusin & David T. Breault & Paula Q. Barrett & Mark P. Beenhakker, 2020. "Angiotensin II induces coordinated calcium bursts in aldosterone-producing adrenal rosettes," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15408-4
    DOI: 10.1038/s41467-020-15408-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-15408-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-15408-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15408-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.