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Cep55 promotes cytokinesis of neural progenitors but is dispensable for most mammalian cell divisions

Author

Listed:
  • Antonio Tedeschi

    (The Francis Crick Institute
    Cancer Research UK London Research Institute)

  • Jorge Almagro

    (The Francis Crick Institute)

  • Matthew J. Renshaw

    (The Francis Crick Institute)

  • Hendrik A. Messal

    (The Francis Crick Institute
    Oncode Institute, Netherlands Cancer Institute)

  • Axel Behrens

    (The Francis Crick Institute
    King’s College London)

  • Mark Petronczki

    (Cancer Research UK London Research Institute
    Boehringer Ingelheim RCV GmbH & Co KG)

Abstract

In mammalian cell lines, the endosomal sorting complex required for transport (ESCRT)-III mediates abscission, the process that physically separates daughter cells and completes cell division. Cep55 protein is regarded as the master regulator of abscission, because it recruits ESCRT-III to the midbody (MB), the site of abscission. However, the importance of this mechanism in a mammalian organism has never been tested. Here we show that Cep55 is dispensable for mouse embryonic development and adult tissue homeostasis. Cep55-knockout offspring show microcephaly and primary neural progenitors require Cep55 and ESCRT for survival and abscission. However, Cep55 is dispensable for cell division in embryonic or adult tissues. In vitro, division of primary fibroblasts occurs without Cep55 and ESCRT-III at the midbody and is not affected by ESCRT depletion. Our work defines Cep55 as an abscission regulator only in specific tissue contexts and necessitates the re-evaluation of an alternative ESCRT-independent cell division mechanism.

Suggested Citation

  • Antonio Tedeschi & Jorge Almagro & Matthew J. Renshaw & Hendrik A. Messal & Axel Behrens & Mark Petronczki, 2020. "Cep55 promotes cytokinesis of neural progenitors but is dispensable for most mammalian cell divisions," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15359-w
    DOI: 10.1038/s41467-020-15359-w
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    Cited by:

    1. Seppe Goovaerts & Hanne Hoskens & Ryan J. Eller & Noah Herrick & Anthony M. Musolf & Cristina M. Justice & Meng Yuan & Sahin Naqvi & Myoung Keun Lee & Dirk Vandermeulen & Heather L. Szabo-Rogers & Pau, 2023. "Joint multi-ancestry and admixed GWAS reveals the complex genetics behind human cranial vault shape," Nature Communications, Nature, vol. 14(1), pages 1-21, December.

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