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An expanded library of orthogonal split inteins enables modular multi-peptide assemblies

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  • Filipe Pinto

    (University of Edinburgh
    University of Edinburgh)

  • Ella Lucille Thornton

    (University of Edinburgh
    University of Edinburgh)

  • Baojun Wang

    (University of Edinburgh
    University of Edinburgh)

Abstract

Inteins are protein segments capable of joining adjacent residues via a peptide bond. In this process known as protein splicing, the intein itself is not present in the final sequence, thus achieving scarless peptide ligation. Here, we assess the splicing activity of 34 inteins (both uncharacterized and known) using a rapid split fluorescent reporter characterization platform, and establish a library of 15 mutually orthogonal split inteins for in vivo applications, 10 of which can be simultaneously used in vitro. We show that orthogonal split inteins can be coupled to multiple split transcription factors to implement complex logic circuits in living organisms, and that they can also be used for the in vitro seamless assembly of large repetitive proteins with biotechnological relevance. Our work demonstrates the versatility and vast potential of an expanded library of orthogonal split inteins for their use in the fields of synthetic biology and protein engineering.

Suggested Citation

  • Filipe Pinto & Ella Lucille Thornton & Baojun Wang, 2020. "An expanded library of orthogonal split inteins enables modular multi-peptide assemblies," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15272-2
    DOI: 10.1038/s41467-020-15272-2
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    Cited by:

    1. Andrew M. King & Daniel A. Anderson & Emerson Glassey & Thomas H. Segall-Shapiro & Zhengan Zhang & David L. Niquille & Amanda C. Embree & Katelin Pratt & Thomas L. Williams & D. Benjamin Gordon & Chri, 2021. "Selection for constrained peptides that bind to a single target protein," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Stanislav Anastassov & Maurice Filo & Ching-Hsiang Chang & Mustafa Khammash, 2023. "A cybergenetic framework for engineering intein-mediated integral feedback control systems," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    3. Yuanli Gao & Lei Wang & Baojun Wang, 2023. "Customizing cellular signal processing by synthetic multi-level regulatory circuits," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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