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Wnt-PLC-IP3-Connexin-Ca2+ axis maintains ependymal motile cilia in zebrafish spinal cord

Author

Listed:
  • Jun Zhang

    (Chonnam National University Medical School
    Center for Creative Biomedical Scientists at Chonnam National University)

  • Gopalakrishnan Chandrasekaran

    (Chonnam National University Medical School
    Center for Creative Biomedical Scientists at Chonnam National University)

  • Wenting Li

    (Chonnam National University Medical School
    Graduate School, Chonnam National University)

  • Dong-Young Kim

    (University of Ulsan College of Medicine)

  • In Young Jeong

    (Korea University)

  • So-Hyun Lee

    (Chonnam National University Medical School
    Center for Creative Biomedical Scientists at Chonnam National University)

  • Ting Liang

    (Chonnam National University Medical School
    Center for Creative Biomedical Scientists at Chonnam National University)

  • Jin Young Bae

    (School of Dentistry, Kyungpook National University)

  • Isaac Choi

    (College of Bioscience and Biotechnology, Chungnam National University)

  • Hyuno Kang

    (Korea Basic Science Institute)

  • Jin-Soo Maeng

    (Korea Food Research Institute
    Korea Institute of Chemical Technology)

  • Myeong-Kyu Kim

    (Chonnam National University Medical School)

  • Taewon Lee

    (College of Science and Technology, Korea University)

  • Seung Woo Park

    (Yonsei University College of Medicine)

  • Min Jung Kim

    (Sookmyung Women’s University)

  • Hyung-Seok Kim

    (Chonnam National University Medical School)

  • Hyunju Ro

    (College of Bioscience and Biotechnology, Chungnam National University)

  • Yong Chul Bae

    (School of Dentistry, Kyungpook National University)

  • Hae-Chul Park

    (Korea University)

  • Eun Young Choi

    (University of Ulsan College of Medicine)

  • Seok-Yong Choi

    (Chonnam National University Medical School
    Center for Creative Biomedical Scientists at Chonnam National University)

Abstract

Ependymal cells (ECs) are multiciliated neuroepithelial cells that line the ventricles of the brain and the central canal of the spinal cord (SC). How ependymal motile cilia are maintained remains largely unexplored. Here we show that zebrafish embryos deficient in Wnt signaling have defective motile cilia, yet harbor intact basal bodies. With respect to maintenance of ependymal motile cilia, plcδ3a is a target gene of Wnt signaling. Lack of Connexin43 (Cx43), especially its channel function, decreases motile cilia and intercellular Ca2+ wave (ICW) propagation. Genetic ablation of cx43 in zebrafish and mice diminished motile cilia. Finally, Cx43 is also expressed in ECs of the human SC. Taken together, our findings indicate that gap junction mediated ICWs play an important role in the maintenance of ependymal motile cilia, and suggest that the enhancement of functional gap junctions by pharmacological or genetic manipulations may be adopted to ameliorate motile ciliopathy.

Suggested Citation

  • Jun Zhang & Gopalakrishnan Chandrasekaran & Wenting Li & Dong-Young Kim & In Young Jeong & So-Hyun Lee & Ting Liang & Jin Young Bae & Isaac Choi & Hyuno Kang & Jin-Soo Maeng & Myeong-Kyu Kim & Taewon , 2020. "Wnt-PLC-IP3-Connexin-Ca2+ axis maintains ependymal motile cilia in zebrafish spinal cord," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15248-2
    DOI: 10.1038/s41467-020-15248-2
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