IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-15180-5.html
   My bibliography  Save this article

SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development

Author

Listed:
  • Lucia Falbo

    (IFOM, The FIRC Institute of Molecular Oncology)

  • Erica Raspelli

    (IFOM, The FIRC Institute of Molecular Oncology)

  • Francesco Romeo

    (IFOM, The FIRC Institute of Molecular Oncology)

  • Simona Fiorani

    (Cancer Research UK)

  • Federica Pezzimenti

    (IFOM, The FIRC Institute of Molecular Oncology)

  • Francesca Casagrande

    (IFOM, The FIRC Institute of Molecular Oncology)

  • Ilaria Costa

    (IFOM, The FIRC Institute of Molecular Oncology)

  • Dario Parazzoli

    (IFOM, The FIRC Institute of Molecular Oncology)

  • Vincenzo Costanzo

    (IFOM, The FIRC Institute of Molecular Oncology
    University of Milan)

Abstract

In several metazoans, the number of active replication origins in embryonic nuclei is higher than in somatic ones, ensuring rapid genome duplication during synchronous embryonic cell divisions. High replication origin density can be restored by somatic nuclear reprogramming. However, mechanisms underlying high replication origin density formation coupled to rapid cell cycles are poorly understood. Here, using Xenopus laevis, we show that SSRP1 stimulates replication origin assembly on somatic chromatin by promoting eviction of histone H1 through its N-terminal domain. Histone H1 removal derepresses ORC and MCM chromatin binding, allowing efficient replication origin assembly. SSRP1 protein decays at mid-blastula transition (MBT) when asynchronous somatic cell cycles start. Increasing levels of SSRP1 delay MBT and, surprisingly, accelerate post-MBT cell cycle speed and embryo development. These findings identify a major epigenetic mechanism regulating DNA replication and directly linking replication origin assembly, cell cycle duration and embryo development in vertebrates.

Suggested Citation

  • Lucia Falbo & Erica Raspelli & Francesco Romeo & Simona Fiorani & Federica Pezzimenti & Francesca Casagrande & Ilaria Costa & Dario Parazzoli & Vincenzo Costanzo, 2020. "SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15180-5
    DOI: 10.1038/s41467-020-15180-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-15180-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-15180-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Lara Djakovic & Thomas Hennig & Katharina Reinisch & Andrea Milić & Adam W. Whisnant & Katharina Wolf & Elena Weiß & Tobias Haas & Arnhild Grothey & Christopher S. Jürges & Michael Kluge & Elmar Wolf , 2023. "The HSV-1 ICP22 protein selectively impairs histone repositioning upon Pol II transcription downstream of genes," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15180-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.