Author
Listed:
- Sabrina Jabs
(Unité des Interactions Bactéries-Cellules, Institut Pasteur, U604 Institut National de la Santé et de la Recherche Médicale, USC 2020 Institut National de la Recherche Agronomique)
- Anne Biton
(Hub de Bioinformatique et Biostatistique – Département Biologie Computationnelle, Institut Pasteur, USR 3756 CNRS)
- Christophe Bécavin
(Hub de Bioinformatique et Biostatistique – Département Biologie Computationnelle, Institut Pasteur, USR 3756 CNRS)
- Marie-Anne Nahori
(Unité des Interactions Bactéries-Cellules, Institut Pasteur, U604 Institut National de la Santé et de la Recherche Médicale, USC 2020 Institut National de la Recherche Agronomique)
- Amine Ghozlane
(Hub de Bioinformatique et Biostatistique – Département Biologie Computationnelle, Institut Pasteur, USR 3756 CNRS)
- Alessandro Pagliuso
(Unité des Interactions Bactéries-Cellules, Institut Pasteur, U604 Institut National de la Santé et de la Recherche Médicale, USC 2020 Institut National de la Recherche Agronomique)
- Giulia Spanò
(Unité des Interactions Bactéries-Cellules, Institut Pasteur, U604 Institut National de la Santé et de la Recherche Médicale, USC 2020 Institut National de la Recherche Agronomique)
- Vincent Guérineau
(Institut de Chimie des Substances Naturelles, CNRS UPR 2301, Université Paris-Sud, Université Paris-Saclay)
- David Touboul
(Institut de Chimie des Substances Naturelles, CNRS UPR 2301, Université Paris-Sud, Université Paris-Saclay)
- Quentin Giai Gianetto
(Hub de Bioinformatique et Biostatistique – Département Biologie Computationnelle, Institut Pasteur, USR 3756 CNRS
Unité de spectrométrie de masse et Protéomique, CNRS USR 2000, Institut Pasteur)
- Thibault Chaze
(Unité de spectrométrie de masse et Protéomique, CNRS USR 2000, Institut Pasteur)
- Mariette Matondo
(Unité de spectrométrie de masse et Protéomique, CNRS USR 2000, Institut Pasteur)
- Marie-Agnès Dillies
(Hub de Bioinformatique et Biostatistique – Département Biologie Computationnelle, Institut Pasteur, USR 3756 CNRS)
- Pascale Cossart
(Unité des Interactions Bactéries-Cellules, Institut Pasteur, U604 Institut National de la Santé et de la Recherche Médicale, USC 2020 Institut National de la Recherche Agronomique)
Abstract
The intestinal microbiota modulates host physiology and gene expression via mechanisms that are not fully understood. Here we examine whether host epitranscriptomic marks are affected by the gut microbiota. We use methylated RNA-immunoprecipitation and sequencing (MeRIP-seq) to identify N6-methyladenosine (m6A) modifications in mRNA of mice carrying conventional, modified, or no microbiota. We find that variations in the gut microbiota correlate with m6A modifications in the cecum, and to a lesser extent in the liver, affecting pathways related to metabolism, inflammation and antimicrobial responses. We analyze expression levels of several known writer and eraser enzymes, and find that the methyltransferase Mettl16 is downregulated in absence of a microbiota, and one of its target mRNAs, encoding S-adenosylmethionine synthase Mat2a, is less methylated. We furthermore show that Akkermansia muciniphila and Lactobacillus plantarum affect specific m6A modifications in mono-associated mice. Our results highlight epitranscriptomic modifications as an additional level of interaction between commensal bacteria and their host.
Suggested Citation
Sabrina Jabs & Anne Biton & Christophe Bécavin & Marie-Anne Nahori & Amine Ghozlane & Alessandro Pagliuso & Giulia Spanò & Vincent Guérineau & David Touboul & Quentin Giai Gianetto & Thibault Chaze & , 2020.
"Impact of the gut microbiota on the m6A epitranscriptome of mouse cecum and liver,"
Nature Communications, Nature, vol. 11(1), pages 1-16, December.
Handle:
RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15126-x
DOI: 10.1038/s41467-020-15126-x
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Cited by:
- Kristin M. Ham & Layne K. Bower & Shanping Li & Hernan Lorenzi & Safiatou Doumbo & Didier Doumtabe & Kassoum Kayentao & Aissata Ongoiba & Boubacar Traore & Peter D. Crompton & Nathan W. Schmidt, 2024.
"The gut microbiome is associated with susceptibility to febrile malaria in Malian children,"
Nature Communications, Nature, vol. 15(1), pages 1-16, December.
- Shujie Chen, & Lu Zhang & Mengjie Li & Ying Zhang & Meng Sun & Lingfang Wang & Jiebo Lin & Yun Cui & Qian Chen & Chenqi Jin & Xiang Li & Boya Wang & Hao Chen & Tianhua Zhou & Liangjing Wang & Chih-Hun, 2022.
"Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis,"
Nature Communications, Nature, vol. 13(1), pages 1-16, December.
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