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Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9

Author

Listed:
  • Oliver Xiaoou Dong

    (University of California
    Innovative Genomics Institute
    The Joint Bioenergy Institute)

  • Shu Yu

    (University of California)

  • Rashmi Jain

    (University of California)

  • Nan Zhang

    (University of California)

  • Phat Q. Duong

    (University of California)

  • Corinne Butler

    (University of California)

  • Yan Li

    (University of California)

  • Anna Lipzen

    (Department of Energy Joint Genome Institute)

  • Joel A. Martin

    (Department of Energy Joint Genome Institute)

  • Kerrie W. Barry

    (Department of Energy Joint Genome Institute)

  • Jeremy Schmutz

    (Department of Energy Joint Genome Institute)

  • Li Tian

    (University of California)

  • Pamela C. Ronald

    (University of California
    Innovative Genomics Institute
    The Joint Bioenergy Institute)

Abstract

Targeted insertion of transgenes at pre-determined plant genomic safe harbors provides a desirable alternative to insertions at random sites achieved through conventional methods. Most existing cases of targeted gene insertion in plants have either relied on the presence of a selectable marker gene in the insertion cassette or occurred at low frequency with relatively small DNA fragments (

Suggested Citation

  • Oliver Xiaoou Dong & Shu Yu & Rashmi Jain & Nan Zhang & Phat Q. Duong & Corinne Butler & Yan Li & Anna Lipzen & Joel A. Martin & Kerrie W. Barry & Jeremy Schmutz & Li Tian & Pamela C. Ronald, 2020. "Marker-free carotenoid-enriched rice generated through targeted gene insertion using CRISPR-Cas9," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14981-y
    DOI: 10.1038/s41467-020-14981-y
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