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Stem rust resistance in wheat is suppressed by a subunit of the mediator complex

Author

Listed:
  • Colin W. Hiebert

    (Agriculture and Agri-Food Canada, Morden Research and Development Centre)

  • Matthew J. Moscou

    (University of East Anglia)

  • Tim Hewitt

    (University of Sydney
    CSIRO Agriculture & Food)

  • Burkhard Steuernagel

    (John Innes Centre, Norwich Research Park)

  • Inma Hernández-Pinzón

    (University of East Anglia)

  • Phon Green

    (University of East Anglia)

  • Vincent Pujol

    (The Australian National University)

  • Peng Zhang

    (University of Sydney)

  • Matthew N. Rouse

    (University of Minnesota)

  • Yue Jin

    (University of Minnesota)

  • Robert A. McIntosh

    (University of Sydney)

  • Narayana Upadhyaya

    (CSIRO Agriculture & Food)

  • Jianping Zhang

    (CSIRO Agriculture & Food)

  • Sridhar Bhavani

    (CIMMYT, ICRAF House, United Nations Avenue)

  • Jan Vrána

    (Centre of the Region Haná for Biotechnological and Agricultural Research)

  • Miroslava Karafiátová

    (Centre of the Region Haná for Biotechnological and Agricultural Research)

  • Li Huang

    (Montana State University)

  • Tom Fetch

    (Agriculture and Agri-Food Canada, Morden Research and Development Centre)

  • Jaroslav Doležel

    (Centre of the Region Haná for Biotechnological and Agricultural Research)

  • Brande B. H. Wulff

    (John Innes Centre, Norwich Research Park)

  • Evans Lagudah

    (CSIRO Agriculture & Food)

  • Wolfgang Spielmeyer

    (CSIRO Agriculture & Food)

Abstract

Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar ‘Canthatch’ suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.

Suggested Citation

  • Colin W. Hiebert & Matthew J. Moscou & Tim Hewitt & Burkhard Steuernagel & Inma Hernández-Pinzón & Phon Green & Vincent Pujol & Peng Zhang & Matthew N. Rouse & Yue Jin & Robert A. McIntosh & Narayana , 2020. "Stem rust resistance in wheat is suppressed by a subunit of the mediator complex," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14937-2
    DOI: 10.1038/s41467-020-14937-2
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    Cited by:

    1. Chuntian Lu & Jie Du & Heyu Chen & Shuangjun Gong & Yinyu Jin & Xiangru Meng & Ting Zhang & Bisheng Fu & István Molnár & Kateřina Holušová & Mahmoud Said & Liping Xing & Lingna Kong & Jaroslav Doležel, 2024. "Wheat Pm55 alleles exhibit distinct interactions with an inhibitor to cause different powdery mildew resistance," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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