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Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells

Author

Listed:
  • Magdalena Wagner

    (Karolinska Institutet and Karolinska University Hospital)

  • Masahito Yoshihara

    (Karolinska Institutet)

  • Iyadh Douagi

    (Karolinska Institutet
    Flow Cytometry Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health)

  • Anastasios Damdimopoulos

    (Bioinformatics and Expression Analysis core facility, Karolinska Institutet)

  • Sarita Panula

    (Karolinska Institutet and Karolinska University Hospital)

  • Sophie Petropoulos

    (Karolinska Institutet and Karolinska University Hospital
    University of Montreal)

  • Haojiang Lu

    (Karolinska Institutet and Karolinska University Hospital)

  • Karin Pettersson

    (Karolinska Institutet and Karolinska University Hospital)

  • Kerstin Palm

    (Karolinska Institutet and Karolinska University Hospital
    Visby hospital)

  • Shintaro Katayama

    (Karolinska Institutet)

  • Outi Hovatta

    (Karolinska Institutet and Karolinska University Hospital)

  • Juha Kere

    (Karolinska Institutet
    University of Helsinki)

  • Fredrik Lanner

    (Karolinska Institutet and Karolinska University Hospital
    Ming Wai Lau Center for Reparative Medicine, Stockholm node, Karolinska Institutet)

  • Pauliina Damdimopoulou

    (Karolinska Institutet and Karolinska University Hospital)

Abstract

The human ovary orchestrates sex hormone production and undergoes monthly structural changes to release mature oocytes. The outer lining of the ovary (cortex) has a key role in defining fertility in women as it harbors the ovarian reserve. It has been postulated that putative oogonial stem cells exist in the ovarian cortex and that these can be captured by DDX4 antibody isolation. Here, we report single-cell transcriptomes and cell surface antigen profiles of over 24,000 cells from high quality ovarian cortex samples from 21 patients. Our data identify transcriptional profiles of six main cell types; oocytes, granulosa cells, immune cells, endothelial cells, perivascular cells, and stromal cells. Cells captured by DDX4 antibody are perivascular cells, not oogonial stem cells. Our data do not support the existence of germline stem cells in adult human ovaries, thereby reinforcing the dogma of a limited ovarian reserve.

Suggested Citation

  • Magdalena Wagner & Masahito Yoshihara & Iyadh Douagi & Anastasios Damdimopoulos & Sarita Panula & Sophie Petropoulos & Haojiang Lu & Karin Pettersson & Kerstin Palm & Shintaro Katayama & Outi Hovatta , 2020. "Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14936-3
    DOI: 10.1038/s41467-020-14936-3
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    Cited by:

    1. Moïra Rossitto & Stephanie Déjardin & Chris M. Rands & Stephanie Gras & Roberta Migale & Mahmoud-Reza Rafiee & Yasmine Neirijnck & Alain Pruvost & Anvi Laetitia Nguyen & Guillaume Bossis & Florence Ca, 2022. "TRIM28-dependent SUMOylation protects the adult ovary from activation of the testicular pathway," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    2. Simeng Hu & Can Hu & Jingli Xu & Pengfei Yu & Li Yuan & Ziyu Li & Haohong Liang & Yanqiang Zhang & Jiahui Chen & Qing Wei & Shengjie Zhang & Litao Yang & Dan Su & Yian Du & Zhiyuan Xu & Fan Bai & Xian, 2024. "The estrogen response in fibroblasts promotes ovarian metastases of gastric cancer," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    3. Ilmatar Rooda & Jasmin Hassan & Jie Hao & Magdalena Wagner & Elisabeth Moussaud-Lamodière & Kersti Jääger & Marjut Otala & Katri Knuus & Cecilia Lindskog & Kiriaki Papaikonomou & Sebastian Gidlöf & Ce, 2024. "In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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