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Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma

Author

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  • Bin Kong

    (Macromolecular Platforms for Translational Medicine and Bio-Manufacturing Laboratory, Tsinghua-Berkeley Shenzhen Institute
    Biomanufacturing Engineering Laboratory, Tsinghua Shenzhen International Graduate School)

  • Yun Chen

    (Tsinghua Shenzhen International Graduate School)

  • Rui Liu

    (Biomanufacturing Engineering Laboratory, Tsinghua Shenzhen International Graduate School)

  • Xi Liu

    (Beijing Children’s Hospital)

  • Changyong Liu

    (Shenzhen University)

  • Zengwu Shao

    (Huazhong University Science & Technology)

  • Liming Xiong

    (Huazhong University Science & Technology)

  • Xianning Liu

    (Shaanxi Institute of Ophthalmology
    Shaanxi Key Laboratory of Eye)

  • Wei Sun

    (Macromolecular Platforms for Translational Medicine and Bio-Manufacturing Laboratory, Tsinghua-Berkeley Shenzhen Institute
    Tsinghua University
    Drexel University)

  • Shengli Mi

    (Biomanufacturing Engineering Laboratory, Tsinghua Shenzhen International Graduate School
    Tsinghua Shenzhen International Graduate School)

Abstract

Regeneration of corneal stroma has always been a challenge due to its sophisticated structure and keratocyte-fibroblast transformation. In this study, we fabricate grid poly (ε-caprolactone)-poly (ethylene glycol) microfibrous scaffold and infuse the scaffold with gelatin methacrylate (GelMA) hydrogel to obtain a 3 D fiber hydrogel construct; the fiber spacing is adjusted to fabricate optimal construct that simulates the stromal structure with properties most similar to the native cornea. The topological structure (3 D fiber hydrogel, 3 D GelMA hydrogel, and 2 D culture dish) and chemical factors (serum, ascorbic acid, insulin, and β-FGF) are examined to study their effects on the differentiation of limbal stromal stem cells to keratocytes or fibroblasts and the phenotype maintenance, in vitro and in vivo tissue regeneration. The results demonstrate that fiber hydrogel and serum-free media synergize to provide an optimal environment for the maintenance of keratocyte phenotype and the regeneration of damaged corneal stroma.

Suggested Citation

  • Bin Kong & Yun Chen & Rui Liu & Xi Liu & Changyong Liu & Zengwu Shao & Liming Xiong & Xianning Liu & Wei Sun & Shengli Mi, 2020. "Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14887-9
    DOI: 10.1038/s41467-020-14887-9
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    Cited by:

    1. Won Bae Han & Gwan-Jin Ko & Kang-Gon Lee & Donghak Kim & Joong Hoon Lee & Seung Min Yang & Dong-Je Kim & Jeong-Woong Shin & Tae-Min Jang & Sungkeun Han & Honglei Zhou & Heeseok Kang & Jun Hyeon Lim & , 2023. "Ultra-stretchable and biodegradable elastomers for soft, transient electronics," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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