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The role of Mediator and Little Elongation Complex in transcription termination

Author

Listed:
  • Hidehisa Takahashi

    (Yokohama City University Graduate School of Medical Science)

  • Amol Ranjan

    (Stowers Institute for Medical Research)

  • Shiyuan Chen

    (Stowers Institute for Medical Research)

  • Hidefumi Suzuki

    (Yokohama City University Graduate School of Medical Science)

  • Mio Shibata

    (Hokkaido University)

  • Tomonori Hirose

    (Yokohama City University Graduate School of Medical Science)

  • Hiroko Hirose

    (Yokohama City University Graduate School of Medical Science)

  • Kazunori Sasaki

    (Yokohama City University Graduate School of Medical Science)

  • Ryota Abe

    (Yokohama City University Graduate School of Medical Science)

  • Kai Chen

    (Stowers Institute for Medical Research)

  • Yanfeng He

    (Stowers Institute for Medical Research)

  • Ying Zhang

    (Stowers Institute for Medical Research)

  • Ichigaku Takigawa

    (Hokkaido University)

  • Tadasuke Tsukiyama

    (Hokkaido University)

  • Masashi Watanabe

    (Hokkaido University)

  • Satoshi Fujii

    (Kyushu Institute of Technology)

  • Midori Iida

    (Kyushu Institute of Technology)

  • Junichi Yamamoto

    (Department of Nanoparticle Translational Research Tokyo Medical University)

  • Yuki Yamaguchi

    (Tokyo Institute of Technology)

  • Yutaka Suzuki

    (The University of Tokyo)

  • Masaki Matsumoto

    (Kyushu University
    Kyushu University)

  • Keiichi I. Nakayama

    (Kyushu University
    Kyushu University)

  • Michael P. Washburn

    (Stowers Institute for Medical Research
    University of Kansas Medical Center)

  • Anita Saraf

    (Stowers Institute for Medical Research)

  • Laurence Florens

    (Stowers Institute for Medical Research)

  • Shigeo Sato

    (Stowers Institute for Medical Research)

  • Chieri Tomomori-Sato

    (Stowers Institute for Medical Research)

  • Ronald C. Conaway

    (Stowers Institute for Medical Research
    University of Kansas Medical Center)

  • Joan W. Conaway

    (Stowers Institute for Medical Research
    University of Kansas Medical Center)

  • Shigetsugu Hatakeyama

    (Hokkaido University)

Abstract

Mediator is a coregulatory complex that regulates transcription of Pol II-dependent genes. Previously, we showed that human Mediator subunit MED26 plays a role in the recruitment of Super Elongation Complex (SEC) or Little Elongation Complex (LEC) to regulate the expression of certain genes. MED26 plays a role in recruiting SEC to protein-coding genes including c-myc and LEC to small nuclear RNA (snRNA) genes. However, how MED26 engages SEC or LEC to regulate distinct genes is unclear. Here, we provide evidence that MED26 recruits LEC to modulate transcription termination of non-polyadenylated transcripts including snRNAs and mRNAs encoding replication-dependent histone (RDH) at Cajal bodies. Our findings indicate that LEC recruited by MED26 promotes efficient transcription termination by Pol II through interaction with CBC-ARS2 and NELF/DSIF, and promotes 3′ end processing by enhancing recruitment of Integrator or Heat Labile Factor to snRNA or RDH genes, respectively.

Suggested Citation

  • Hidehisa Takahashi & Amol Ranjan & Shiyuan Chen & Hidefumi Suzuki & Mio Shibata & Tomonori Hirose & Hiroko Hirose & Kazunori Sasaki & Ryota Abe & Kai Chen & Yanfeng He & Ying Zhang & Ichigaku Takigawa, 2020. "The role of Mediator and Little Elongation Complex in transcription termination," Nature Communications, Nature, vol. 11(1), pages 1-20, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14849-1
    DOI: 10.1038/s41467-020-14849-1
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    Cited by:

    1. Hidefumi Suzuki & Ryota Abe & Miho Shimada & Tomonori Hirose & Hiroko Hirose & Keisuke Noguchi & Yoko Ike & Nanami Yasui & Kazuki Furugori & Yuki Yamaguchi & Atsushi Toyoda & Yutaka Suzuki & Tatsuro Y, 2022. "The 3′ Pol II pausing at replication-dependent histone genes is regulated by Mediator through Cajal bodies’ association with histone locus bodies," Nature Communications, Nature, vol. 13(1), pages 1-24, December.

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