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Precise timing of transcription by c-di-GMP coordinates cell cycle and morphogenesis in Caulobacter

Author

Listed:
  • Andreas Kaczmarczyk

    (University of Basel)

  • Antje M. Hempel

    (University of Basel)

  • Christoph Arx

    (University of Basel
    Interregional Blood Transfusion SRC Ltd.)

  • Raphael Böhm

    (University of Basel)

  • Badri N. Dubey

    (University of Basel)

  • Jutta Nesper

    (University of Basel)

  • Tilman Schirmer

    (University of Basel)

  • Sebastian Hiller

    (University of Basel)

  • Urs Jenal

    (University of Basel)

Abstract

Bacteria adapt their growth rate to their metabolic status and environmental conditions by modulating the length of their G1 period. Here we demonstrate that a gradual increase in the concentration of the second messenger c-di-GMP determines precise gene expression during G1/S transition in Caulobacter crescentus. We show that c-di-GMP stimulates the kinase ShkA by binding to its central pseudo-receiver domain, activates the TacA transcription factor, and initiates a G1/S-specific transcription program leading to cell morphogenesis and S-phase entry. Activation of the ShkA-dependent genetic program causes c-di-GMP to reach peak levels, which triggers S-phase entry and promotes proteolysis of ShkA and TacA. Thus, a gradual increase of c-di-GMP results in precise control of ShkA-TacA activity, enabling G1/S-specific gene expression that coordinates cell cycle and morphogenesis.

Suggested Citation

  • Andreas Kaczmarczyk & Antje M. Hempel & Christoph Arx & Raphael Böhm & Badri N. Dubey & Jutta Nesper & Tilman Schirmer & Sebastian Hiller & Urs Jenal, 2020. "Precise timing of transcription by c-di-GMP coordinates cell cycle and morphogenesis in Caulobacter," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14585-6
    DOI: 10.1038/s41467-020-14585-6
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    Cited by:

    1. Mitchell Brüderlin & Raphael Böhm & Firas Fadel & Sebastian Hiller & Tilman Schirmer & Badri N. Dubey, 2023. "Structural features discriminating hybrid histidine kinase Rec domains from response regulator homologs," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Andreas Kaczmarczyk & Simon Vliet & Roman Peter Jakob & Raphael Dias Teixeira & Inga Scheidat & Alberto Reinders & Alexander Klotz & Timm Maier & Urs Jenal, 2024. "A genetically encoded biosensor to monitor dynamic changes of c-di-GMP with high temporal resolution," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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