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Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome

Author

Listed:
  • Daniel Fox

    (The Australian National University)

  • Anukriti Mathur

    (The Australian National University)

  • Yansong Xue

    (The Australian National University)

  • Yunqi Liu

    (The Australian National University)

  • Wei Hong Tan

    (The Australian National University)

  • Shouya Feng

    (The Australian National University)

  • Abhimanu Pandey

    (The Australian National University)

  • Chinh Ngo

    (The Australian National University)

  • Jenni A. Hayward

    (The Australian National University)

  • Ines I. Atmosukarto

    (The Australian National University)

  • Jason D. Price

    (The Australian National University)

  • Matthew D. Johnson

    (The Australian National University)

  • Nadja Jessberger

    (Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität München)

  • Avril A. B. Robertson

    (The University of Queensland)

  • Gaetan Burgio

    (The Australian National University)

  • David C. Tscharke

    (The Australian National University)

  • Edward M. Fox

    (Northumbria University)

  • Denisse L. Leyton

    (The Australian National University
    The Australian National University)

  • Nadeem O. Kaakoush

    (UNSW Sydney)

  • Erwin Märtlbauer

    (Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität München)

  • Stephen H. Leppla

    (Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health)

  • Si Ming Man

    (The Australian National University)

Abstract

Inflammasomes are important for host defence against pathogens and homeostasis with commensal microbes. Here, we show non-haemolytic enterotoxin (NHE) from the neglected human foodborne pathogen Bacillus cereus is an activator of the NLRP3 inflammasome and pyroptosis. NHE is a non-redundant toxin to haemolysin BL (HBL) despite having a similar mechanism of action. Via a putative transmembrane region, subunit C of NHE initiates binding to the plasma membrane, leading to the recruitment of subunit B and subunit A, thus forming a tripartite lytic pore that is permissive to efflux of potassium. NHE mediates killing of cells from multiple lineages and hosts, highlighting a versatile functional repertoire in different host species. These data indicate that NHE and HBL operate synergistically to induce inflammation and show that multiple virulence factors from the same pathogen with conserved function and mechanism of action can be exploited for sensing by a single inflammasome.

Suggested Citation

  • Daniel Fox & Anukriti Mathur & Yansong Xue & Yunqi Liu & Wei Hong Tan & Shouya Feng & Abhimanu Pandey & Chinh Ngo & Jenni A. Hayward & Ines I. Atmosukarto & Jason D. Price & Matthew D. Johnson & Nadja, 2020. "Bacillus cereus non-haemolytic enterotoxin activates the NLRP3 inflammasome," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14534-3
    DOI: 10.1038/s41467-020-14534-3
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    Cited by:

    1. Shouya Feng & Daniel Enosi Tuipulotu & Abhimanu Pandey & Weidong Jing & Cheng Shen & Chinh Ngo & Melkamu B. Tessema & Fei-Ju Li & Daniel Fox & Anukriti Mathur & Anyang Zhao & Runli Wang & Klaus Pfeffe, 2022. "Pathogen-selective killing by guanylate-binding proteins as a molecular mechanism leading to inflammasome signaling," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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