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Ultracompact 3D microfluidics for time-resolved structural biology

Author

Listed:
  • Juraj Knoška

    (Deutsches Elektronen-Synchrotron DESY
    Universität Hamburg)

  • Luigi Adriano

    (Deutsches Elektronen-Synchrotron
    EuXFEL, Sample Environment & Characterization Group)

  • Salah Awel

    (Deutsches Elektronen-Synchrotron DESY
    Universität Hamburg)

  • Kenneth R. Beyerlein

    (Deutsches Elektronen-Synchrotron DESY
    Max Planck Institute for the Structure and Dynamics of Matter)

  • Oleksandr Yefanov

    (Deutsches Elektronen-Synchrotron DESY)

  • Dominik Oberthuer

    (Deutsches Elektronen-Synchrotron DESY)

  • Gisel E. Peña Murillo

    (Deutsches Elektronen-Synchrotron DESY
    Universität Hamburg)

  • Nils Roth

    (Deutsches Elektronen-Synchrotron DESY
    Universität Hamburg)

  • Iosifina Sarrou

    (Deutsches Elektronen-Synchrotron DESY)

  • Pablo Villanueva-Perez

    (Deutsches Elektronen-Synchrotron DESY
    Lund University)

  • Max O. Wiedorn

    (Deutsches Elektronen-Synchrotron DESY
    Universität Hamburg)

  • Fabian Wilde

    (Institut für Werkstoffforschung)

  • Saša Bajt

    (Deutsches Elektronen-Synchrotron)

  • Henry N. Chapman

    (Deutsches Elektronen-Synchrotron DESY
    Universität Hamburg
    Universität Hamburg)

  • Michael Heymann

    (Deutsches Elektronen-Synchrotron DESY
    Universität Stuttgart)

Abstract

To advance microfluidic integration, we present the use of two-photon additive manufacturing to fold 2D channel layouts into compact free-form 3D fluidic circuits with nanometer precision. We demonstrate this technique by tailoring microfluidic nozzles and mixers for time-resolved structural biology at X-ray free-electron lasers (XFELs). We achieve submicron jets with speeds exceeding 160 m s−1, which allows for the use of megahertz XFEL repetition rates. By integrating an additional orifice, we implement a low consumption flow-focusing nozzle, which is validated by solving a hemoglobin structure. Also, aberration-free in operando X-ray microtomography is introduced to study efficient equivolumetric millisecond mixing in channels with 3D features integrated into the nozzle. Such devices can be printed in minutes by locally adjusting print resolution during fabrication. This technology has the potential to permit ultracompact devices and performance improvements through 3D flow optimization in all fields of microfluidic engineering.

Suggested Citation

  • Juraj Knoška & Luigi Adriano & Salah Awel & Kenneth R. Beyerlein & Oleksandr Yefanov & Dominik Oberthuer & Gisel E. Peña Murillo & Nils Roth & Iosifina Sarrou & Pablo Villanueva-Perez & Max O. Wiedorn, 2020. "Ultracompact 3D microfluidics for time-resolved structural biology," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14434-6
    DOI: 10.1038/s41467-020-14434-6
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