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White blood cell and cell-free DNA analyses for detection of residual disease in gastric cancer

Author

Listed:
  • Alessandro Leal

    (Johns Hopkins University School of Medicine)

  • Nicole C. T. van Grieken

    (Vrije Universiteit)

  • Doreen N. Palsgrove

    (Johns Hopkins University School of Medicine)

  • Jillian Phallen

    (Johns Hopkins University School of Medicine)

  • Jamie E. Medina

    (Johns Hopkins University School of Medicine)

  • Carolyn Hruban

    (Johns Hopkins University School of Medicine)

  • Mark A. M. Broeckaert

    (Vrije Universiteit)

  • Valsamo Anagnostou

    (Johns Hopkins University School of Medicine)

  • Vilmos Adleff

    (Johns Hopkins University School of Medicine)

  • Daniel C. Bruhm

    (Johns Hopkins University School of Medicine)

  • Jenna V. Canzoniero

    (Johns Hopkins University School of Medicine)

  • Jacob Fiksel

    (Johns Hopkins University School of Medicine)

  • Marianne Nordsmark

    (Aarhus University Hospital)

  • Fabienne A. R. M. Warmerdam

    (Zuyderland Medical Centre)

  • Henk M. W. Verheul

    (Vrije Universiteit)

  • Dick Johan van Spronsen

    (Radboud University Nijmegen Medical Centre)

  • Laurens V. Beerepoot

    (St. Elisabeth-Tweesteden Ziekenhuis)

  • Maud M. Geenen

    (Onze Lieve Vrouwe Gasthuis)

  • Johanneke E. A. Portielje

    (HAGA hospital
    Leiden University Medical Center)

  • Edwin P. M. Jansen

    (Netherlands Cancer Institute)

  • Johanna van Sandick

    (Netherlands Cancer Institute)

  • Elma Meershoek-Klein Kranenbarg

    (Leiden University Medical Center)

  • Hanneke W. M. van Laarhoven

    (Amsterdam UMC)

  • Donald L. van der Peet

    (Vrije Universiteit)

  • Cornelis J. H. van de Velde

    (Leiden University Medical Center)

  • Marcel Verheij

    (Netherlands Cancer Institute)

  • Remond Fijneman

    (Netherlands Cancer Institute)

  • Robert B. Scharpf

    (Johns Hopkins University School of Medicine)

  • Gerrit A. Meijer

    (Netherlands Cancer Institute)

  • Annemieke Cats

    (Netherlands Cancer Institute)

  • Victor E. Velculescu

    (Johns Hopkins University School of Medicine)

Abstract

Liquid biopsies are providing new opportunities for detection of residual disease in cell-free DNA (cfDNA) after surgery but may be confounded through identification of alterations arising from clonal hematopoiesis. Here, we identify circulating tumor-derived DNA (ctDNA) alterations through ultrasensitive targeted sequencing analyses of matched cfDNA and white blood cells from the same patient. We apply this approach to analyze samples from patients in the CRITICS trial, a phase III randomized controlled study of perioperative treatment in patients with operable gastric cancer. After filtering alterations from matched white blood cells, the presence of ctDNA predicts recurrence when analyzed within nine weeks after preoperative treatment and after surgery in patients eligible for multimodal treatment. These analyses provide a facile method for distinguishing ctDNA from other cfDNA alterations and highlight the utility of ctDNA as a predictive biomarker of patient outcome to perioperative cancer therapy and surgical resection in patients with gastric cancer.

Suggested Citation

  • Alessandro Leal & Nicole C. T. van Grieken & Doreen N. Palsgrove & Jillian Phallen & Jamie E. Medina & Carolyn Hruban & Mark A. M. Broeckaert & Valsamo Anagnostou & Vilmos Adleff & Daniel C. Bruhm & J, 2020. "White blood cell and cell-free DNA analyses for detection of residual disease in gastric cancer," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14310-3
    DOI: 10.1038/s41467-020-14310-3
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    Cited by:

    1. Iris van ’t Erve & Bahar Alipanahi & Keith Lumbard & Zachary L. Skidmore & Lorenzo Rinaldi & Laurel K. Millberg & Jacob Carey & Bryan Chesnick & Stephen Cristiano & Carter Portwood & Tony Wu & Erica P, 2024. "Cancer treatment monitoring using cell-free DNA fragmentomes," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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