IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-14299-9.html
   My bibliography  Save this article

Hepatitis Delta Virus histone mimicry drives the recruitment of chromatin remodelers for viral RNA replication

Author

Listed:
  • Natali Abeywickrama-Samarakoon

    (INSERM, U1052 UMR CNRS 5286, Cancer Research Center of Lyon (CRCL))

  • Jean-Claude Cortay

    (INSERM, U1052 UMR CNRS 5286, Cancer Research Center of Lyon (CRCL))

  • Camille Sureau

    (Laboratoire de Virologie Moléculaire, INSERM U1134, Institut National de la Transfusion Sanguine)

  • Susanne Müller

    (Structural Genomics Consortium, Buchmann Institute for Molecular Life Sciences, Johann Wolfgang Goethe-University)

  • Dulce Alfaiate

    (INSERM, U1052 UMR CNRS 5286, Cancer Research Center of Lyon (CRCL)
    Université de Genève
    Hôpital de la Croix Rousse, Hospices Civils de Lyon and Université Lyon I)

  • Francesca Guerrieri

    (INSERM, U1052 UMR CNRS 5286, Cancer Research Center of Lyon (CRCL)
    Sapienza University)

  • Apirat Chaikuad

    (Structural Genomics Consortium, Buchmann Institute for Molecular Life Sciences, Johann Wolfgang Goethe-University)

  • Martin Schröder

    (Structural Genomics Consortium, Buchmann Institute for Molecular Life Sciences, Johann Wolfgang Goethe-University)

  • Philippe Merle

    (INSERM, U1052 UMR CNRS 5286, Cancer Research Center of Lyon (CRCL)
    Hôpital de la Croix Rousse, Hospices Civils de Lyon and Université Lyon I)

  • Massimo Levrero

    (INSERM, U1052 UMR CNRS 5286, Cancer Research Center of Lyon (CRCL)
    Sapienza University
    Hôpital de la Croix Rousse, Hospices Civils de Lyon and Université Lyon I)

  • Paul Dény

    (INSERM, U1052 UMR CNRS 5286, Cancer Research Center of Lyon (CRCL)
    Laboratoire de Microbiologie Clinique, Groupe des Hôpitaux Universitaires de Paris - Seine Saint Denis, UFR Santé Médecine, Biologie Humaine, Université Paris 13)

Abstract

Hepatitis Delta virus (HDV) is a satellite of Hepatitis B virus with a single-stranded circular RNA genome. HDV RNA genome synthesis is carried out in infected cells by cellular RNA polymerases with the assistance of the small hepatitis delta antigen (S-HDAg). Here we show that S-HDAg binds the bromodomain (BRD) adjacent to zinc finger domain 2B (BAZ2B) protein, a regulatory subunit of BAZ2B-associated remodeling factor (BRF) ISWI chromatin remodeling complexes. shRNA-mediated silencing of BAZ2B or its inactivation with the BAZ2B BRD inhibitor GSK2801 impairs HDV replication in HDV-infected human hepatocytes. S-HDAg contains a short linear interacting motif (SLiM) KacXXR, similar to the one recognized by BAZ2B BRD in histone H3. We found that the integrity of the S-HDAg SLiM sequence is required for S-HDAg interaction with BAZ2B BRD and for HDV RNA replication. Our results suggest that S-HDAg uses a histone mimicry strategy to co-activate the RNA polymerase II-dependent synthesis of HDV RNA and sustain HDV replication.

Suggested Citation

  • Natali Abeywickrama-Samarakoon & Jean-Claude Cortay & Camille Sureau & Susanne Müller & Dulce Alfaiate & Francesca Guerrieri & Apirat Chaikuad & Martin Schröder & Philippe Merle & Massimo Levrero & Pa, 2020. "Hepatitis Delta Virus histone mimicry drives the recruitment of chromatin remodelers for viral RNA replication," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14299-9
    DOI: 10.1038/s41467-020-14299-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-14299-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-14299-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-14299-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.