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FICD activity and AMPylation remodelling modulate human neurogenesis

Author

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  • Pavel Kielkowski

    (Technical University of Munich)

  • Isabel Y. Buchsbaum

    (Max Planck Institute of Psychiatry
    Graduate School of Systemic Neurosciences, Ludwig-Maximilians-University Munich)

  • Volker C. Kirsch

    (Technical University of Munich)

  • Nina C. Bach

    (Technical University of Munich)

  • Micha Drukker

    (Helmholtz Center)

  • Silvia Cappello

    (Max Planck Institute of Psychiatry)

  • Stephan A. Sieber

    (Technical University of Munich)

Abstract

Posttranslational modification (PTM) of proteins represents an important cellular mechanism for controlling diverse functions such as signalling, localisation or protein–protein interactions. AMPylation (also termed adenylylation) has recently been discovered as a prevalent PTM for regulating protein activity. In human cells AMPylation has been exclusively studied with the FICD protein. Here we investigate the role of AMPylation in human neurogenesis by introducing a cell-permeable propargyl adenosine pronucleotide probe to infiltrate cellular AMPylation pathways and report distinct modifications in intact cancer cell lines, human-derived stem cells, neural progenitor cells (NPCs), neurons and cerebral organoids (COs) via LC–MS/MS as well as imaging methods. A total of 162 AMP modified proteins were identified. FICD-dependent AMPylation remodelling accelerates differentiation of neural progenitor cells into mature neurons in COs, demonstrating a so far unknown trigger of human neurogenesis.

Suggested Citation

  • Pavel Kielkowski & Isabel Y. Buchsbaum & Volker C. Kirsch & Nina C. Bach & Micha Drukker & Silvia Cappello & Stephan A. Sieber, 2020. "FICD activity and AMPylation remodelling modulate human neurogenesis," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14235-6
    DOI: 10.1038/s41467-019-14235-6
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    Cited by:

    1. Dandan Wang & Lingfang Zhu & Xiangkai Zhen & Daoyan Yang & Changfu Li & Yating Chen & Huannan Wang & Yichen Qu & Xiaozhen Liu & Yanling Yin & Huawei Gu & Lei Xu & Chuanxing Wan & Yao Wang & Songying O, 2022. "A secreted effector with a dual role as a toxin and as a transcriptional factor," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Yizhi Yuan & Florian M. Stumpf & Lisa A. Schlor & Olivia P. Schmidt & Philip Saumer & Luisa B. Huber & Matthias Frese & Eva Höllmüller & Martin Scheffner & Florian Stengel & Kay Diederichs & Andreas M, 2023. "Chemoproteomic discovery of a human RNA ligase," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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