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Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA

Author

Listed:
  • Wooyoung Kang

    (Seoul National University)

  • Kook Sun Ha

    (Korea Advanced Institute of Science and Technology
    University of Suwon)

  • Heesoo Uhm

    (Seoul National University
    University of Oxford)

  • Kyuhyong Park

    (Seoul National University)

  • Ja Yil Lee

    (Ulsan National Institute of Science and Technology)

  • Sungchul Hohng

    (Seoul National University)

  • Changwon Kang

    (Korea Advanced Institute of Science and Technology)

Abstract

Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the essential components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from the DNA template much more often than their concurrent dissociations in intrinsic termination of bacterial transcription. As termination is defined by the release of product RNA from the transcription complex, the subsequent retention of RNAP on DNA constitutes a previously unidentified stage, termed here as recycling. During the recycling stage, post-terminational RNAPs one-dimensionally diffuse on DNA in downward and upward directions, and can initiate transcription again at the original and nearby promoters in the case of retaining a sigma factor. The efficiency of this event, termed here as reinitiation, increases with supplement of a sigma factor. In summary, after releasing RNA product at intrinsic termination, recycling RNAP diffuses on the DNA template for reinitiation most of the time.

Suggested Citation

  • Wooyoung Kang & Kook Sun Ha & Heesoo Uhm & Kyuhyong Park & Ja Yil Lee & Sungchul Hohng & Changwon Kang, 2020. "Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14200-3
    DOI: 10.1038/s41467-019-14200-3
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    Cited by:

    1. Eunho Song & Heesoo Uhm & Palinda Ruvan Munasingha & Seungha Hwang & Yeon-Soo Seo & Jin Young Kang & Changwon Kang & Sungchul Hohng, 2022. "Rho-dependent transcription termination proceeds via three routes," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Jin Qian & Bing Wang & Irina Artsimovitch & David Dunlap & Laura Finzi, 2024. "Force and the α-C-terminal domains bias RNA polymerase recycling," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    3. Ying Xiong & Weijing Han & Chunhua Xu & Jing Shi & Lisha Wang & Taoli Jin & Qi Jia & Ying Lu & Shuxin Hu & Shuo-Xing Dou & Wei Lin & Terence R. Strick & Shuang Wang & Ming Li, 2024. "Single-molecule reconstruction of eukaryotic factor-dependent transcription termination," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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