IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-019-14058-5.html
   My bibliography  Save this article

Single cell and genetic analyses reveal conserved populations and signaling mechanisms of gastrointestinal stromal niches

Author

Listed:
  • Ji-Eun Kim

    (The Hospital for Sick Children, Toronto
    University of Toronto, Toronto)

  • Lijiang Fei

    (Zhejiang University of School of Medicine)

  • Wen-Chi Yin

    (The Hospital for Sick Children, Toronto
    University of Toronto, Toronto)

  • Sabrina Coquenlorge

    (The Hospital for Sick Children, Toronto
    University of Toronto, Toronto)

  • Abilasha Rao-Bhatia

    (The Hospital for Sick Children, Toronto
    University of Toronto, Toronto)

  • Xiaoyun Zhang

    (The Hospital for Sick Children, Toronto
    University of Toronto, Toronto)

  • Sammy Shun Wai Shi

    (The Hospital for Sick Children, Toronto
    University of Toronto)

  • Ju Hee Lee

    (The Hospital for Sick Children, Toronto
    University of Toronto)

  • Noah A. Hahn

    (The Hospital for Sick Children, Toronto
    University of Toronto, Toronto)

  • Wasi Rizvi

    (The Hospital for Sick Children, Toronto
    University of Toronto, Toronto)

  • Kyoung-Han Kim

    (University of Ottawa Heart Institute, Ottawa
    University of Ottawa, Ottawa)

  • Hoon-Ki Sung

    (The Hospital for Sick Children, Toronto
    University of Toronto)

  • Chi-chung Hui

    (The Hospital for Sick Children, Toronto
    University of Toronto, Toronto)

  • Guoji Guo

    (Zhejiang University of School of Medicine)

  • Tae-Hee Kim

    (The Hospital for Sick Children, Toronto
    University of Toronto, Toronto)

Abstract

Stomach and intestinal stem cells are located in discrete niches called the isthmus and crypt, respectively. Recent studies have demonstrated a surprisingly conserved role for Wnt signaling in gastrointestinal development. Although intestinal stromal cells secrete Wnt ligands to promote stem cell renewal, the source of stomach Wnt ligands is still unclear. Here, by performing single cell analysis, we identify gastrointestinal stromal cell populations with transcriptome signatures that are conserved between the stomach and intestine. In close proximity to epithelial cells, these perictye-like cells highly express telocyte and pericyte markers as well as Wnt ligands, and they are enriched for Hh signaling. By analyzing mice activated for Hh signaling, we show a conserved mechanism of GLI2 activation of Wnt ligands. Moreover, genetic inhibition of Wnt secretion in perictye-like stromal cells or stromal cells more broadly demonstrates their essential roles in gastrointestinal regeneration and development, respectively, highlighting a redundancy in gastrointestinal stem cell niches.

Suggested Citation

  • Ji-Eun Kim & Lijiang Fei & Wen-Chi Yin & Sabrina Coquenlorge & Abilasha Rao-Bhatia & Xiaoyun Zhang & Sammy Shun Wai Shi & Ju Hee Lee & Noah A. Hahn & Wasi Rizvi & Kyoung-Han Kim & Hoon-Ki Sung & Chi-c, 2020. "Single cell and genetic analyses reveal conserved populations and signaling mechanisms of gastrointestinal stromal niches," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14058-5
    DOI: 10.1038/s41467-019-14058-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-14058-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-14058-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Jeremiah Bernier-Latmani & Cristina Mauri & Rachel Marcone & François Renevey & Stephan Durot & Liqun He & Michael Vanlandewijck & Catherine Maclachlan & Suzel Davanture & Nicola Zamboni & Graham W. K, 2022. "ADAMTS18+ villus tip telocytes maintain a polarized VEGFA signaling domain and fenestrations in nutrient-absorbing intestinal blood vessels," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Hitomi Takada & Yohei Sasagawa & Mika Yoshimura & Kaori Tanaka & Yoshimi Iwayama & Tetsutaro Hayashi & Ayako Isomura-Matoba & Itoshi Nikaido & Akira Kurisaki, 2023. "Single-cell transcriptomics uncovers EGFR signaling-mediated gastric progenitor cell differentiation in stomach homeostasis," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    3. Ryan J. Smith & Minggao Liang & Adrian Kwan Ho Loe & Theodora Yung & Ji-Eun Kim & Matthew Hudson & Michael D. Wilson & Tae-Hee Kim, 2023. "Epigenetic control of cellular crosstalk defines gastrointestinal organ fate and function," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    4. Elisa Manieri & Guodong Tie & Ermanno Malagola & Davide Seruggia & Shariq Madha & Adrianna Maglieri & Kun Huang & Yuko Fujiwara & Kevin Zhang & Stuart H. Orkin & Timothy C. Wang & Ruiyang He & Neil Mc, 2023. "Role of PDGFRA+ cells and a CD55+ PDGFRALo fraction in the gastric mesenchymal niche," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    5. Naveen Kumar & Pon Ganish Prakash & Christian Wentland & Shilpa Mary Kurian & Gaurav Jethva & Volker Brinkmann & Hans-Joachim Mollenkopf & Tobias Krammer & Christophe Toussaint & Antoine-Emmanuel Sali, 2024. "Decoding spatiotemporal transcriptional dynamics and epithelial fibroblast crosstalk during gastroesophageal junction development through single cell analysis," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    6. Marta Kapalczynska & Manqiang Lin & Jeroen Maertzdorf & Julian Heuberger & Stefanie Muellerke & Xiangsheng Zuo & Ramon Vidal & Imad Shureiqi & Anne-Sophie Fischer & Sascha Sauer & Hilmar Berger & Evel, 2022. "BMP feed-forward loop promotes terminal differentiation in gastric glands and is interrupted by H. pylori-driven inflammation," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14058-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.