IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-019-14042-z.html
   My bibliography  Save this article

Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages

Author

Listed:
  • Aurélie Mathieu

    (Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute)

  • Moïra Dion

    (Université Laval
    Université Laval)

  • Ling Deng

    (University of Copenhagen)

  • Denise Tremblay

    (Université Laval
    Université Laval)

  • Elisabeth Moncaut

    (Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute)

  • Shiraz A. Shah

    (Copenhagen University Hospital)

  • Jakob Stokholm

    (Copenhagen University Hospital)

  • Karen A. Krogfelt

    (Statens Serum Institut)

  • Susanne Schjørring

    (Statens Serum Institut)

  • Hans Bisgaard

    (Copenhagen University Hospital)

  • Dennis S. Nielsen

    (University of Copenhagen)

  • Sylvain Moineau

    (Université Laval
    Université Laval
    Université Laval)

  • Marie-Agnès Petit

    (Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute)

Abstract

Bacteriophages constitute an important part of the human gut microbiota, but their impact on this community is largely unknown. Here, we cultivate temperate phages produced by 900 E. coli strains isolated from 648 fecal samples from 1-year-old children and obtain coliphages directly from the viral fraction of the same fecal samples. We find that 63% of strains hosted phages, while 24% of the viromes contain phages targeting E. coli. 150 of these phages, half recovered from strain supernatants, half from virome (73% temperate and 27% virulent) were tested for their host range on 75 E. coli strains isolated from the same cohort. Temperate phages barely infected the gut strains, whereas virulent phages killed up to 68% of them. We conclude that in fecal samples from children, temperate coliphages dominate, while virulent ones have greater infectivity and broader host range, likely playing a role in gut microbiota dynamics.

Suggested Citation

  • Aurélie Mathieu & Moïra Dion & Ling Deng & Denise Tremblay & Elisabeth Moncaut & Shiraz A. Shah & Jakob Stokholm & Karen A. Krogfelt & Susanne Schjørring & Hans Bisgaard & Dennis S. Nielsen & Sylvain , 2020. "Virulent coliphages in 1-year-old children fecal samples are fewer, but more infectious than temperate coliphages," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14042-z
    DOI: 10.1038/s41467-019-14042-z
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-019-14042-z
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-019-14042-z?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Diana P. Baquero & Sofia Medvedeva & Camille Martin-Gallausiaux & Nika Pende & Anna Sartori-Rupp & Stéphane Tachon & Thierry Pedron & Laurent Debarbieux & Guillaume Borrel & Simonetta Gribaldo & Mart , 2024. "Stable coexistence between an archaeal virus and the dominant methanogen of the human gut," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14042-z. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.