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Non-invasive optical control of endogenous Ca2+ channels in awake mice

Author

Listed:
  • Sungsoo Kim

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Taeyoon Kyung

    (Institute for Basic Science (IBS)
    Massachusetts Institute of Technology)

  • Jae-Hee Chung

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Nury Kim

    (Institute for Basic Science (IBS))

  • Sehoon Keum

    (Institute for Basic Science (IBS))

  • Jinsu Lee

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Hyerim Park

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Ho Min Kim

    (Institute for Basic Science (IBS)
    Korea Advanced Institute of Science and Technology (KAIST))

  • Sangkyu Lee

    (Institute for Basic Science (IBS))

  • Hee-Sup Shin

    (Institute for Basic Science (IBS))

  • Won Do Heo

    (Korea Advanced Institute of Science and Technology (KAIST)
    Institute for Basic Science (IBS)
    Korea Advanced Institute of Science and Technology (KAIST))

Abstract

Optogenetic approaches for controlling Ca2+ channels provide powerful means for modulating diverse Ca2+-specific biological events in space and time. However, blue light-responsive photoreceptors are, in principle, considered inadequate for deep tissue stimulation unless accompanied by optic fiber insertion. Here, we present an ultra-light-sensitive optogenetic Ca2+ modulator, named monSTIM1 encompassing engineered cryptochrome2 for manipulating Ca2+ signaling in the brain of awake mice through non-invasive light delivery. Activation of monSTIM1 in either excitatory neurons or astrocytes of mice brain is able to induce Ca2+-dependent gene expression without any mechanical damage in the brain. Furthermore, we demonstrate that non-invasive Ca2+ modulation in neurons can be sufficiently and effectively translated into changes in behavioral phenotypes of awake mice.

Suggested Citation

  • Sungsoo Kim & Taeyoon Kyung & Jae-Hee Chung & Nury Kim & Sehoon Keum & Jinsu Lee & Hyerim Park & Ho Min Kim & Sangkyu Lee & Hee-Sup Shin & Won Do Heo, 2020. "Non-invasive optical control of endogenous Ca2+ channels in awake mice," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-019-14005-4
    DOI: 10.1038/s41467-019-14005-4
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    Cited by:

    1. Yaling Dou & Rui Chen & Siyao Liu & Yi-Tsang Lee & Ji Jing & Xiaoxuan Liu & Yuepeng Ke & Rui Wang & Yubin Zhou & Yun Huang, 2023. "Optogenetic engineering of STING signaling allows remote immunomodulation to enhance cancer immunotherapy," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Rodrigo G. Fernandez Lahore & Niccolò P. Pampaloni & Enrico Schiewer & M.-Marcel Heim & Linda Tillert & Johannes Vierock & Johannes Oppermann & Jakob Walther & Dietmar Schmitz & David Owald & Andrew J, 2022. "Calcium-permeable channelrhodopsins for the photocontrol of calcium signalling," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

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